Immobilization and stabilization of microbial lipases by multipoint covalent attachment on aldehyde-resin affinity: Application of the biocatalysts in biodiesel synthesis


Autoria(s): MENDES, Adriano A.; GIORDANO, Roberto C.; GIORDANO, Raquel de L. C.; CASTRO, Heizir F. de
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/10/2012

18/10/2012

2011

Resumo

Microbial lipase preparations from Thermomyces lanuginosus (TLL) and Pseudomonas fluorescens (PFL) were immobilized by multipoint covalent attachment on Toyopearl AF-amino-650M resin and the most active and thermal stable derivatives used to catalyze the transesterificanon reaction of babassu and palm oils with ethanol in solvent-free media For this different activating agents mainly glutaraldehyde glycidol and epichlorohydrin were used and immobilization parameters were estimated based on the hydrolysis of olive oil emulsion and butyl butyrate synthesis ILL immobilized on glyoxyl-resin allowed obtaining derivatives with the highest hydrolytic activity (HA(der)) and thermal stability between 27 and 31 times more stable than the soluble lipase Although PFL derivatives were found to be less active and thermally stables similar formation of butyl butyrate concentrations were found for both ILL and PFL derivatives The highest conversion into biodiesel was found in the transesterification of palm oil catalyzed by both ILL and PFL glyoxyl-derivatives (c) 2010 Elsevier B V All rights reserved

FAPESP (Brazil)[04/14593-4]

Identificador

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, v.68, n.1, p.109-115, 2011

1381-1177

http://producao.usp.br/handle/BDPI/17548

10.1016/j.molcatb.2010.10.002

http://dx.doi.org/10.1016/j.molcatb.2010.10.002

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Journal of Molecular Catalysis B-enzymatic

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Multipoint covalent attachment #Lipases #Resin affinity #Thermal-stability #Biodiesel #ACTIVATED SUPPORTS #BACTERIAL LIPASES #GLYOXYL-AGAROSE #CHITOSAN #COMPOSITE #PROTEINS #ENZYMES #SILICA #FATS #GELS #Biochemistry & Molecular Biology #Chemistry, Physical
Tipo

article

original article

publishedVersion