In vitro organogenesis of Citrus volkameriana and Citrus aurantium


Autoria(s): TAVANO, E. C. R.; STIPP, L. C. L.; MUNIZ, F. R.; MOURAO FILHO, F. A. A.; MENDES, B. M. J.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/10/2012

18/10/2012

2009

Resumo

In vitro organogenesis of Citrus volkameriana and C. aurantium was studied considering three explant types: epicotyl segment, internodal segment, and hypocotyl segment with attached cotyledon fragment. The explants were cultured in medium according to Grosser and Gmitter (EME) supplemented with 0, 0.5, 1.0, 1.5, and 2.0 mg dm(- 3) 6-benzyl-aminopurine (BAP), incubated firstly in darkness for 4 weeks, and then transferred to 16-h photoperiod for 2 weeks. Comparing epicotyl and internodal segments, a higher percentage of responsive explants and a higher number of shoots per explant were obtained with epicotyl segments, regardless of the BAP concentration. For C. volkameriana the highest percentage of responsive epicotyl segments (42 %) was obtained in EME with 1.0 mg dm(-3) BAP, while for C. aurantium (59 %) in EME with 0.5 mg dm(-3) BAP. The organogenesis efficiency was the best with the use of the hypocotyl segment with attached cotyledon fragment (77 % for C. volkameriana and to 75 % for C. aurantium). With this explant the morphogenesis occurred only in the hypocotyl region. The in vitro organogenesis was characterized by histological analyses showing that the morphogenic process started in the cambium region near the explant cut end.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)

Fundo de Defesa da Citricultura (Fundecitrus)

ECRT and FRM

Identificador

BIOLOGIA PLANTARUM, v.53, n.2, p.395-399, 2009

0006-3134

http://producao.usp.br/handle/BDPI/16981

10.1007/s10535-009-0075-2

http://dx.doi.org/10.1007/s10535-009-0075-2

Idioma(s)

eng

Publicador

SPRINGER

Relação

Biologia Plantarum

Direitos

restrictedAccess

Copyright SPRINGER

Palavras-Chave #adventitious bud #BAP #epicotyl #hypocotyl #internodal segment #sour orange #volkamer lemon #AGROBACTERIUM-MEDIATED TRANSFORMATION #TROYER CITRANGE #EPICOTYL CUTTINGS #EFFICIENT REGENERATION #MORPHOGENIC RESPONSE #EXPLANT ORIENTATION #SHOOT REGENERATION #SWEET ORANGE #SEGMENTS #TISSUE #Plant Sciences
Tipo

article

original article

publishedVersion