Identification of COL6A1 as a differentially expressed gene in human astrocytomas


Autoria(s): FUJITA, A.; SATO, J. R.; FESTA, F.; GOMES, L. R.; OBA-SHINJO, S. M.; MARIE, S. K. N.; FERREIRA, C. E.; SOGAYAR, M. C.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

19/04/2012

19/04/2012

2008

Resumo

Diffuse infiltrating gliomas are the most common tumors of the central nervous system. Gliomas are classified by the WHO according to their histopathological and clinical characteristics into four classes: grade I (pilocytic astrocytoma), grade II (diffuse astrocytoma), grade III (anaplastic astrocytoma), and grade IV (glioblastoma multiforme). Several genes have already been correlated with astrocytomas, but many others are yet to be uncovered. By analyzing the public SAGE data from 21 patients, comprising low malignant grade astrocytomas and glioblastomas, we found COL6A1 to be differentially expressed, confirming this finding by real time RT-PCR in 66 surgical samples. To the best of our knowledge, COL6A1 has never been described in gliomas. The expression of this gene has significantly different means when normal glia is compared with low-grade astrocytomas (grades I and II) and high-grade astrocytomas (grades III and IV), with a tendency to be greater in higher grade samples, thus rendering it a powerful tumor marker.

Identificador

GENETICS AND MOLECULAR RESEARCH, v.7, n.2, p.371-378, 2008

1676-5680

http://producao.usp.br/handle/BDPI/16653

http://www.geneticsmr.com//year2008/vol7-2/pdf/gmr432.pdf

Idioma(s)

eng

Publicador

FUNPEC-EDITORA

Relação

Genetics and Molecular Research

Direitos

openAccess

Copyright FUNPEC-EDITORA

Palavras-Chave #COL6A1 gene #astrocytomas #differential gene expression #tumor marker #SAGE #SUPPRESSION SUBTRACTIVE HYBRIDIZATION #POLYMERASE CHAIN-REACTION #CENTRAL-NERVOUS-SYSTEM #MICROARRAY ANALYSIS #GLIOMAS #TUMORS #CLASSIFICATION #GLIOBLASTOMA #PROFILES #GRADE #Biochemistry & Molecular Biology #Genetics & Heredity
Tipo

article

original article

publishedVersion