Purification, crystallization and preliminary crystallographic analysis of the catalytic domain of the extracellular cellulase CBHI from Trichoderma harzianum

The filamentous fungus Trichoderma harzianum has a considerable cellulolytic activity that is mediated by a complex of enzymes which are essential for the hydrolysis of microcrystalline cellulose. These enzymes were produced by the induction of T. harzianum with microcrystalline cellulose (Avicel) under submerged fermentation in a bioreactor. The catalytic core domain (CCD) of cellobiohydrolase I (CBHI) was purified from the extracellular extracts and submitted to robotic crystallization. Diffraction-quality CBHI CCD crystals were grown and an X-ray diffraction data set was collected under cryogenic conditions using a synchrotron-radiation source.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[08/56255-9]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[2007/08706-9]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[2009/05349-6]

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Capes)

Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) via INCT do Bioetanol[471834/2009-2]