Mesenchymal Stem Cells Derived From Canine Umbilical Cord Vein-A Novel Source for Cell Therapy Studies
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
18/04/2012
18/04/2012
2010
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Resumo |
The canine model provides a large animal system to evaluate many treatment modalities using stem cells (SCs). However, only bone marrow ( BM) protocols have been widely used in dogs for preclinical approaches. BM donation consists of an invasive procedure and the number and differentiation potential of its mesenchymal stem cells (MSCs) decline with age. More recently, umbilical cord was introduced as an alternative source to BM since it is obtained from a sample that is routinely discarded. Here, we describe the isolation of MSCs from canine umbilical cord vein (cUCV). These cells can be obtained from every cord received and grow successfully in culture. Their multipotent plasticity was demonstrated by their capacity to differentiate in adipocytic, chondrocytic, and osteocytic lineages. Furthermore, our results open possibilities to use cUCV cells in preclinical trials for many well-characterized canine model conditions homologs to human diseases. CEPID-FAPESP (Centro de Pesquisa, Inovacao e Difusao-Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) INCT (Instituto Nacional de Celulas-tronco em Doencas Geneticas) |
Identificador |
STEM CELLS AND DEVELOPMENT, v.19, n.3, p.395-402, 2010 1547-3287 http://producao.usp.br/handle/BDPI/15537 10.1089/scd.2008.0314 |
Idioma(s) |
eng |
Publicador |
MARY ANN LIEBERT INC |
Relação |
Stem Cells and Development |
Direitos |
closedAccess Copyright MARY ANN LIEBERT INC |
Palavras-Chave | #BONE-MARROW #ADIPOSE-TISSUE #BLOOD #TRANSPLANTATION #MODEL #DOGS #EFFICACY #HOMOLOG #DISEASE #Cell & Tissue Engineering #Hematology #Medicine, Research & Experimental #Transplantation |
Tipo |
article original article publishedVersion |