Mesenchymal Stem Cells Derived From Canine Umbilical Cord Vein-A Novel Source for Cell Therapy Studies


Autoria(s): ZUCCONI, Eder; VIEIRA, Natassia M.; BUENO, Daniela F.; SECCO, Mariane; JAZEDJE, Tatiana; AMBROSIO, Carlos E.; PASSOS-BUENO, Maria Rita; MIGLINO, Maria Angelica; ZATZ, Mayana
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/04/2012

18/04/2012

2010

Resumo

The canine model provides a large animal system to evaluate many treatment modalities using stem cells (SCs). However, only bone marrow ( BM) protocols have been widely used in dogs for preclinical approaches. BM donation consists of an invasive procedure and the number and differentiation potential of its mesenchymal stem cells (MSCs) decline with age. More recently, umbilical cord was introduced as an alternative source to BM since it is obtained from a sample that is routinely discarded. Here, we describe the isolation of MSCs from canine umbilical cord vein (cUCV). These cells can be obtained from every cord received and grow successfully in culture. Their multipotent plasticity was demonstrated by their capacity to differentiate in adipocytic, chondrocytic, and osteocytic lineages. Furthermore, our results open possibilities to use cUCV cells in preclinical trials for many well-characterized canine model conditions homologs to human diseases.

CEPID-FAPESP (Centro de Pesquisa, Inovacao e Difusao-Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)

CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)

INCT (Instituto Nacional de Celulas-tronco em Doencas Geneticas)

Identificador

STEM CELLS AND DEVELOPMENT, v.19, n.3, p.395-402, 2010

1547-3287

http://producao.usp.br/handle/BDPI/15537

10.1089/scd.2008.0314

http://dx.doi.org/10.1089/scd.2008.0314

Idioma(s)

eng

Publicador

MARY ANN LIEBERT INC

Relação

Stem Cells and Development

Direitos

closedAccess

Copyright MARY ANN LIEBERT INC

Palavras-Chave #BONE-MARROW #ADIPOSE-TISSUE #BLOOD #TRANSPLANTATION #MODEL #DOGS #EFFICACY #HOMOLOG #DISEASE #Cell & Tissue Engineering #Hematology #Medicine, Research & Experimental #Transplantation
Tipo

article

original article

publishedVersion