Effect of Low-Level Laser Therapy on Malondialdehyde Concentration in Random Cutaneous Flap Viability
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
18/04/2012
18/04/2012
2010
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Resumo |
Objective: The aim of this study was to assess the effects of 830 and 670 nm laser on malondialdehyde (MDA) concentration in random skin-flap survival. Background Data: Low-level laser therapy (LLLT) has been reported to be successful in stimulating the formation of new blood vessels and activating superoxide-dismutase delivery, thus helping the inhibition of free-radical action and consequently reducing necrosis. Materials and Methods: Thirty Wistar rats were used and divided into three groups, with 10 rats in each one. A random skin flap was raised on the dorsum of each animal. Group 1 was the control group; group 2 received 830 nm laser radiation; and group 3 was submitted to 670 nm laser radiation. The animals underwent laser therapy with 36 J/cm(2) energy density immediately after surgery and on the 4 days subsequent to surgery. The application site of the laser radiation was 1 point, 2.5 cm from the flap's cranial base. The percentage of the skin-flap necrosis area was calculated 7 days postoperative using the paper-template method, and a skin sample was collected immediately after as a way of determining the MDA concentration. Results: Statistically significant differences were found between the necrosis percentages, with higher values seen in group 1 compared with groups 2 and 3. Groups 2 and 3 did not present statistically significant differences (p > 0.05). Group 3 had a lower concentration of MDA values compared to the control group (p < 0.05). Conclusion: LLLT was effective in increasing the random skin-flap viability in rats, and the 670 nm laser was efficient in reducing the MDA concentration. CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) FAEPA University of Sao Paulo (USP) |
Identificador |
PHOTOMEDICINE AND LASER SURGERY, v.28, n.3, p.379-384, 2010 1549-5418 http://producao.usp.br/handle/BDPI/15288 10.1089/pho.2009.2535 |
Idioma(s) |
eng |
Publicador |
MARY ANN LIEBERT INC |
Relação |
Photomedicine and Laser Surgery |
Direitos |
closedAccess Copyright MARY ANN LIEBERT INC |
Palavras-Chave | #HELIUM-NEON LASER #SKIN-FLAP #SURVIVAL #RATS #INJURY #IRRADIATION #Surgery |
Tipo |
article original article publishedVersion |