Effect of Low-Level Laser Therapy on Malondialdehyde Concentration in Random Cutaneous Flap Viability


Autoria(s): PRADO, Rodrigo; NEVES, Lais; MARCOLINO, Alexandre; RIBEIRO, Tiago; PINFILDI, Carlos; FERREIRA, Lydia; THOMAZINI, Jose; PICCINATO, Carlos
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/04/2012

18/04/2012

2010

Resumo

Objective: The aim of this study was to assess the effects of 830 and 670 nm laser on malondialdehyde (MDA) concentration in random skin-flap survival. Background Data: Low-level laser therapy (LLLT) has been reported to be successful in stimulating the formation of new blood vessels and activating superoxide-dismutase delivery, thus helping the inhibition of free-radical action and consequently reducing necrosis. Materials and Methods: Thirty Wistar rats were used and divided into three groups, with 10 rats in each one. A random skin flap was raised on the dorsum of each animal. Group 1 was the control group; group 2 received 830 nm laser radiation; and group 3 was submitted to 670 nm laser radiation. The animals underwent laser therapy with 36 J/cm(2) energy density immediately after surgery and on the 4 days subsequent to surgery. The application site of the laser radiation was 1 point, 2.5 cm from the flap's cranial base. The percentage of the skin-flap necrosis area was calculated 7 days postoperative using the paper-template method, and a skin sample was collected immediately after as a way of determining the MDA concentration. Results: Statistically significant differences were found between the necrosis percentages, with higher values seen in group 1 compared with groups 2 and 3. Groups 2 and 3 did not present statistically significant differences (p > 0.05). Group 3 had a lower concentration of MDA values compared to the control group (p < 0.05). Conclusion: LLLT was effective in increasing the random skin-flap viability in rats, and the 670 nm laser was efficient in reducing the MDA concentration.

CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)

FAEPA

University of Sao Paulo (USP)

Identificador

PHOTOMEDICINE AND LASER SURGERY, v.28, n.3, p.379-384, 2010

1549-5418

http://producao.usp.br/handle/BDPI/15288

10.1089/pho.2009.2535

http://dx.doi.org/10.1089/pho.2009.2535

Idioma(s)

eng

Publicador

MARY ANN LIEBERT INC

Relação

Photomedicine and Laser Surgery

Direitos

closedAccess

Copyright MARY ANN LIEBERT INC

Palavras-Chave #HELIUM-NEON LASER #SKIN-FLAP #SURVIVAL #RATS #INJURY #IRRADIATION #Surgery
Tipo

article

original article

publishedVersion