Adipose Tissue-Derived Stem Cells from Humans and Mice Differ in Proliferative Capacity and Genome Stability in Long-Term Cultures


Autoria(s): DANOVIZ, Maria Elena; BASSANEZE, Vinicius; NAKAMUTA, Juliana Sanajotti; SANTOS-JUNIOR, Gabriel Ribeiro dos; SAINT-CLAIR, Danilo; BAJGELMAN, Marcio Chaim; FAE, Kellen Cristhina; KALIL, Jorge; MIYAKAWA, Ayumi Aurea; KRIEGER, Jose Eduardo
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/04/2012

18/04/2012

2011

Resumo

Adipose tissue-derived stem cells (ASCs) are among the more attractive adult stem cell options for potential therapeutic applications. Here, we studied and compared the basic biological characteristics of ASCs isolated from humans (hASCs) and mice (mASCs) and maintained in identical culture conditions, which must be examined prior to considering further potential clinical applications. hASCs and mASCs were compared for immunophenotype, differentiation potential, cell growth characteristics, senescence, nuclear morphology, and DNA content. Although both strains of ASCs displayed a similar immunophenotype, the percentage of CD73(+) cells was markedly lower and CD31(+) was higher in mASC than in hASC cultures. The mean population doubling time was 98.08 +/- 6.15 h for hASCs and 52.58 +/- 3.74 h for mASCs. The frequency of nuclear aberrations was noticeably lower in hASCs than in mASCs regardless of the passage number. Moreover, as the cells went through several in vitro passages, mASCs showed changes in DNA content and cell cycle kinetics (frequency of hypodiploid, G0/G1, G2/M, and hyperdiploid cells), whereas all of these parameters remained constant in hASCs. Collectively, these results suggest that mASCs display higher proliferative capacity and are more unstable than hASCs in long-term cultures. These results underscore the need to consider specificities among model systems that may influence outcomes when designing potential human applications.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[01/0009-0]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[05/54695-3]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[05/57591-4]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[04/06784-4]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[06/57937-0]

Ministerio da Ciencia e Tecnologia/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/Ministerio da Saude/Departamento Ciencia e Tecnologia (MCT/CNPq/MS/DE-CIT)[552324/20005-1]

Ministerio da Ciencia e Tecnologia/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/Ministerio da Saude/Departamento Ciencia e Tecnologia (MCT/CNPq/MS/DE-CIT)[10120104096700]

Identificador

STEM CELLS AND DEVELOPMENT, v.20, n.4, p.661-670, 2011

1547-3287

http://producao.usp.br/handle/BDPI/15241

10.1089/scd.2010.0231

http://dx.doi.org/10.1089/scd.2010.0231

Idioma(s)

eng

Publicador

MARY ANN LIEBERT INC

Relação

Stem Cells and Development

Direitos

closedAccess

Copyright MARY ANN LIEBERT INC

Palavras-Chave #MARROW STROMAL CELLS #GFP TRANSGENIC MICE #BONE-MARROW #IN-VITRO #GENE-EXPRESSION #RETINOIC ACID #LIFE-SPAN #DIFFERENTIATION #GROWTH #VIVO #Cell & Tissue Engineering #Hematology #Medicine, Research & Experimental #Transplantation
Tipo

article

original article

publishedVersion