Frequency and genotypic distribution of GB virus C (GBV-C) among Colombian population with Hepatitis B (HBV) or Hepatitis C (HCV) infection


Autoria(s): ALVARADO-MORA, Monica V.; Lima, Livia de Souza Botelho; NISHIYA, Anna; AZEVEDO NETO, Raymundo; GOMES GOUVEA, Michele S.; GUTIERREZ, Maria F.; CARRILHO, Flair J.; PINHO, Joao R. R.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/04/2012

18/04/2012

2011

Resumo

Background: GB virus C (GBV-C) is an enveloped positive-sense ssRNA virus belonging to the Flaviviridae family. Studies on the genetic variability of the GBV-C reveals the existence of six genotypes: genotype 1 predominates in West Africa, genotype 2 in Europe and America, genotype 3 in Asia, genotype 4 in Southwest Asia, genotype 5 in South Africa and genotype 6 in Indonesia. The aim of this study was to determine the frequency and genotypic distribution of GBV-C in the Colombian population. Methods: Two groups were analyzed: i) 408 Colombian blood donors infected with HCV (n = 250) and HBV (n = 158) from Bogota and ii) 99 indigenous people with HBV infection from Leticia, Amazonas. A fragment of 344 bp from the 5' untranslated region (5' UTR) was amplified by nested RT PCR. Viral sequences were genotyped by phylogenetic analysis using reference sequences from each genotype obtained from GenBank (n = 160). Bayesian phylogenetic analyses were conducted using Markov chain Monte Carlo (MCMC) approach to obtain the MCC tree using BEAST v. 1.5.3. Results: Among blood donors, from 158 HBsAg positive samples, eight 5.06% (n = 8) were positive for GBV-C and from 250 anti-HCV positive samples, 3.2%(n = 8) were positive for GBV-C. Also, 7.7% (n = 7) GBV-C positive samples were found among indigenous people from Leticia. A phylogenetic analysis revealed the presence of the following GBV-C genotypes among blood donors: 2a (41.6%), 1 (33.3%), 3 (16.6%) and 2b (8.3%). All genotype 1 sequences were found in co-infection with HBV and 4/5 sequences genotype 2a were found in co-infection with HCV. All sequences from indigenous people from Leticia were classified as genotype 3. The presence of GBV-C infection was not correlated with the sex (p = 0.43), age (p = 0.38) or origin (p = 0.17). Conclusions: It was found a high frequency of GBV-C genotype 1 and 2 in blood donors. The presence of genotype 3 in indigenous population was previously reported from Santa Marta region in Colombia and in native people from Venezuela and Bolivia. This fact may be correlated to the ancient movements of Asian people to South America a long time ago.

CNPq

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP[2007/53457-7]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP[2008/50461-6]

Pontificia Universidad Javeriana, Bogota, Colombia

Identificador

Virology Journal, London, v.8(1), 2011

1743-422X

http://producao.usp.br/handle/BDPI/15193

10.1186/1743-422X-8-345

http://dx.doi.org/10.1186/1743-422X-8-345

Idioma(s)

eng

Publicador

BIOMED CENTRAL LTD

LONDON

Relação

Virology Journal

Direitos

openAccess

Copyright BIOMED CENTRAL LTD

Palavras-Chave #C/HEPATITIS-G-VIRUS #POLYMERASE-CHAIN-REACTION #BLOOD-DONORS #HIGH PREVALENCE #MOLECULAR CHARACTERIZATION #HEMODIALYSIS-PATIENTS #PHYLOGENETIC ANALYSIS #REGION SEQUENCES #NONCODING REGION #LIVER-DISEASE #Virology
Tipo

article

original article

publishedVersion