Unexpected Diversity of Cellular Immune Responses against Nef and Vif in HIV-1-Infected Patients Who Spontaneously Control Viral Replication


Autoria(s): TAROSSO, Leandro F.; SAUER, Mariana M.; SANABANI, Sabri; GIRET, Maria Teresa; TOMIYAMA, Helena I.; SIDNEY, John; PIASKOWSKI, Shari M.; DIAZ, Ricardo S.; SABINO, Ester C.; SETTE, Alessandro; KALIL-FILHO, Jorge; WATKINS, David I.; KALLAS, Esper G.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

18/04/2012

18/04/2012

2010

Resumo

Background: HIV-1-infected individuals who spontaneously control viral replication represent an example of successful containment of the AIDS virus. Understanding the anti-viral immune responses in these individuals may help in vaccine design. However, immune responses against HIV-1 are normally analyzed using HIV-1 consensus B 15-mers that overlap by 11 amino acids. Unfortunately, this method may underestimate the real breadth of the cellular immune responses against the autologous sequence of the infecting virus. Methodology and Principal Findings: Here we compared cellular immune responses against nef and vif-encoded consensus B 15-mer peptides to responses against HLA class I-predicted minimal optimal epitopes from consensus B and autologous sequences in six patients who have controlled HIV-1 replication. Interestingly, our analysis revealed that three of our patients had broader cellular immune responses against HLA class I-predicted minimal optimal epitopes from either autologous viruses or from the HIV-1 consensus B sequence, when compared to responses against the 15-mer HIV-1 type B consensus peptides. Conclusion and Significance: This suggests that the cellular immune responses against HIV-1 in controller patients may be broader than we had previously anticipated.

National Institutes of Health (NIH)[R24 RR015371]

Ministry of Health[914/BRA/3014-UNESCO]

Sao Paulo City Health Department[2004-0.168.922-7]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[04/15856-9]

Coordenacao de Aperfeicoamento de Pessoal de Ni-vel Superior (CAPES), Brazilian Ministry of Education

Identificador

PLOS ONE, v.5, n.7, 2010

1932-6203

http://producao.usp.br/handle/BDPI/15085

10.1371/journal.pone.0011436

http://dx.doi.org/10.1371/journal.pone.0011436

Idioma(s)

eng

Publicador

PUBLIC LIBRARY SCIENCE

Relação

Plos One

Direitos

openAccess

Copyright PUBLIC LIBRARY SCIENCE

Palavras-Chave #IMMUNODEFICIENCY-VIRUS TYPE-1 #CD8(+) T-CELLS #HIV-INFECTION #IN-VIVO #NONPROGRESSORS #PEPTIDE #VIREMIA #PROTEIN #GENE #HLA #Biology #Multidisciplinary Sciences
Tipo

article

original article

publishedVersion