Time-resolved fluorescence spectroscopy of white-spot caries in human enamel


Autoria(s): OLIVEIRA, Fernanda Ferretti de; ITO, Amando Siuiti; BACHMANN, Luciano
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

17/04/2012

17/04/2012

2010

Resumo

The objective is to differentiate noncavitated caries enamel through time-resolved fluorescence and to find excitation and emission parameters that can be applied in future clinical practice for detection of caries lesions that are not clearly visible to the professional. Sixteen human teeth with noncavitiated white-spot caries were selected for this work. Fluorescence intensity decay was measured by using an apparatus based on the time-correlated single-photon counting method. An optical fiber bundle was employed for sample excitation (440 nm), and the fluorescence collected by the same bundle (500 nm) was registered. The average lifetime for sound enamel was 7: 93 +/- 0: 09, 2: 46 +/- 0: 04, and 0: 51 +/- 0: 02 ns, whereas for the carious enamel the lifetimes were 4: 84 +/- 0: 06, 1: 35 +/- 0: 02, and 0: 16 +/- 0: 01 ns. It was concluded that it is possible to differentiate between carious and sound regions by time-resolved fluorescence and that, although the origin of enamel fluorescence is still uncertain, the lifetime values seem to be typical of fluorophores like collagen I. (C) 2010 Optical Society of America

Brazilian agencies FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)

CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)

Identificador

APPLIED OPTICS, v.49, n.12, p.2244-2249, 2010

0003-6935

http://producao.usp.br/handle/BDPI/14981

http://www.opticsinfobase.org/view_article.cfm?gotourl=http%3A%2F%2Fwww.opticsinfobase.org%2FDirectPDFAccess%2FF3151E3A-0F32-CF40-7DA98C8E60014FBD_198011.pdf%3Fda%3D1%26id%3D198011%26seq%3D0%26mobile%3Dno&org=Universidad%20de%20Sao%20Paulo%20Instituto%20de%20Fisica%20Biblioteca

Idioma(s)

eng

Publicador

OPTICAL SOC AMER

Relação

Applied Optics

Direitos

openAccess

Copyright OPTICAL SOC AMER

Palavras-Chave #DENTAL-CARIES #DIAGNOSIS #Optics
Tipo

article

original article

publishedVersion