Dendritic Cells Transfected with scFv from Mab 7.B12 Mimicking Original Antigen gp43 Induces Protection against Experimental Paracoccidioidomycosis


Autoria(s): FERREIRA, Karen S.; MARANHAO, Andrea Q.; GARCIA, Maria C. C.; BRIGIDO, Marcelo M.; SANTOS, Suelen S.; LOPES, Jose D.; ALMEIDA, Sandro R.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

17/04/2012

17/04/2012

2011

Resumo

Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naive T cells, and they also participate in Th-cell education. Furthermore, these cells have been used for therapy in several disease models. Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain variable fragment (scFv) of an anti-Id antibody that is capable of mimicking gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein was presented to the regional lymph nodes cells. Moreover, we showed that the DCs transfected with scFv were capable of efficiently activating proliferation of total lymph node cells and inducing a decrease in lung infection. Therefore, our results suggested that the use of scFv-transfected DCs may be a promising therapy in the paracoccidioidomycosis (PCM) model.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[07/57594-9]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[07/50568-2]

CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)

Identificador

PLOS ONE, v.6, n.1, 2011

1932-6203

http://producao.usp.br/handle/BDPI/14815

10.1371/journal.pone.0015935

http://dx.doi.org/10.1371/journal.pone.0015935

Idioma(s)

eng

Publicador

PUBLIC LIBRARY SCIENCE

Relação

Plos One

Direitos

openAccess

Copyright PUBLIC LIBRARY SCIENCE

Palavras-Chave #SINGLE-CHAIN FV #CYTOTOXIC T-LYMPHOCYTES #MARROW-DERIVED CELLS #CARCINOEMBRYONIC ANTIGEN #PLASMID DNA #ANTIIDIOTYPIC ANTIBODIES #IDIOTYPIC ANTIBODIES #DISPLAY LIBRARY #INTERNAL IMAGE #GENE GUN #Biology #Multidisciplinary Sciences
Tipo

article

original article

publishedVersion