Dendritic Cells Transfected with scFv from Mab 7.B12 Mimicking Original Antigen gp43 Induces Protection against Experimental Paracoccidioidomycosis
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
17/04/2012
17/04/2012
2011
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Resumo |
Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naive T cells, and they also participate in Th-cell education. Furthermore, these cells have been used for therapy in several disease models. Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain variable fragment (scFv) of an anti-Id antibody that is capable of mimicking gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein was presented to the regional lymph nodes cells. Moreover, we showed that the DCs transfected with scFv were capable of efficiently activating proliferation of total lymph node cells and inducing a decrease in lung infection. Therefore, our results suggested that the use of scFv-transfected DCs may be a promising therapy in the paracoccidioidomycosis (PCM) model. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[07/57594-9] Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)[07/50568-2] CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) |
Identificador |
PLOS ONE, v.6, n.1, 2011 1932-6203 http://producao.usp.br/handle/BDPI/14815 10.1371/journal.pone.0015935 |
Idioma(s) |
eng |
Publicador |
PUBLIC LIBRARY SCIENCE |
Relação |
Plos One |
Direitos |
openAccess Copyright PUBLIC LIBRARY SCIENCE |
Palavras-Chave | #SINGLE-CHAIN FV #CYTOTOXIC T-LYMPHOCYTES #MARROW-DERIVED CELLS #CARCINOEMBRYONIC ANTIGEN #PLASMID DNA #ANTIIDIOTYPIC ANTIBODIES #IDIOTYPIC ANTIBODIES #DISPLAY LIBRARY #INTERNAL IMAGE #GENE GUN #Biology #Multidisciplinary Sciences |
Tipo |
article original article publishedVersion |