Evaluation of a simple hyphenated system for flow injection solid-phase pre-concentration and capillary electrophoresis

In this work, the development and evaluation of a hyphenated flow injection-capillary electrophoresis system with on-line pre-concentration is described. Preliminary tests were performed to investigate the influence of flow rates over the analytical signals. Results revealed losses in terms of sensitivity of the FIA-CE system when compared to the conventional CE system. To overcome signal decrease and to make the system more efficient, a lower flow rate was set and an anionic resin column was added to the flow manifold in order to pre-concentrate the analyte. The pre-concentration FIA-CE system presented a sensitivity improvement of about 660% and there was only a small increase of 8% in total peak dispersion. These results have confirmed the great potential of the proposed system for many analytical tasks especially for low concentration samples.

Nesse trabalho apresentamos um sistema hifenizado envolvendo as duas técnicas analíticas: eletroforese capilar (CE) e análises por injeção em fluxo (FIA), denominado de FIA-CE. Parâmetros como a influência do fluxo e a natureza do eluente frente ao sistema CE foram avaliados. O sistema demonstrou-se promissor quanto à possibilidade de aplicações em diferentes áreas da química analítica. O aumento da sensibilidade foi da ordem de 660% devido ao emprego de colunas de troca iônica no sistema FIA. Esse resultado comprova o grande potencial do sistema FIA-CE.

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)

CNPq

FAPESP