Application Of Laser Microdissection Icp-ms For High Resolution Elemental Mapping In Mouse Brain Tissue: A Comparative Study With Laser Ablation Icp-ms.


Autoria(s): Sussulini, Alessandra; Becker, J Sabine
Contribuinte(s)

UNIVERSIDADE DE ESTADUAL DE CAMPINAS

Data(s)

01/01/2015

27/11/2015

27/11/2015

Resumo

Mapping of elements in biological tissue by laser induced mass spectrometry is a fast growing analytical methodology in life sciences. This method provides a multitude of useful information of metal, nonmetal, metalloid and isotopic distribution at major, minor and trace concentration ranges, usually with a lateral resolution of 12-160 µm. Selected applications in medical research require an improved lateral resolution of laser induced mass spectrometric technique at the low micrometre scale and below. The present work demonstrates the applicability of a recently developed analytical methodology - laser microdissection associated to inductively coupled plasma mass spectrometry (LMD ICP-MS) - to obtain elemental images of different solid biological samples at high lateral resolution. LMD ICP-MS images of mouse brain tissue samples stained with uranium and native are shown, and a direct comparison of LMD and laser ablation (LA) ICP-MS imaging methodologies, in terms of elemental quantification, is performed.

132

579-82

Identificador

Talanta. v. 132, p. 579-82, 2015-Jan.

1873-3573

10.1016/j.talanta.2014.10.001

http://www.ncbi.nlm.nih.gov/pubmed/25476347

http://repositorio.unicamp.br/jspui/handle/REPOSIP/202319

25476347

Idioma(s)

eng

Relação

Talanta

Talanta

Direitos

fechado

Copyright © 2014 Elsevier B.V. All rights reserved.

Fonte

PubMed

Palavras-Chave #Inductively Coupled Plasma Mass Spectrometry #Laser Ablation #Laser Microdissection #Mass Spectrometry Imaging #Mouse Brain #Quantification
Tipo

Artigo de periódico