A Metabolomic Protocol For Plant Systematics By Matrix-assisted Laser-desorption/ionization Time-of Flight Mass Spectrometry.


Autoria(s): Ernst, Madeleine; Silva, Denise B; Silva, Ricardo; Monge, Marcelo; Semir, João; Vêncio, Ricardo Z N; Lopes, Norberto P
Contribuinte(s)

UNIVERSIDADE DE ESTADUAL DE CAMPINAS

Data(s)

01/02/2015

27/11/2015

27/11/2015

Resumo

Matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) has been widely used for the identification and classification of microorganisms based on their proteomic fingerprints. However, the use of MALDI-TOF MS in plant research has been very limited. In the present study, a first protocol is proposed for metabolic fingerprinting by MALDI-TOF MS using three different MALDI matrices with subsequent multivariate data analysis by in-house algorithms implemented in the R environment for the taxonomic classification of plants from different genera, families and orders. By merging the data acquired with different matrices, different ionization modes and using careful algorithms and parameter selection, we demonstrate that a close taxonomic classification can be achieved based on plant metabolic fingerprints, with 92% similarity to the taxonomic classifications found in literature. The present work therefore highlights the great potential of applying MALDI-TOF MS for the taxonomic classification of plants and, furthermore, provides a preliminary foundation for future research.

859

46-58

Identificador

Analytica Chimica Acta. v. 859, p. 46-58, 2015-Feb.

1873-4324

10.1016/j.aca.2015.01.002

http://www.ncbi.nlm.nih.gov/pubmed/25622605

http://repositorio.unicamp.br/jspui/handle/REPOSIP/202071

25622605

Idioma(s)

eng

Relação

Analytica Chimica Acta

Anal. Chim. Acta

Direitos

fechado

Copyright © 2015 Elsevier B.V. All rights reserved.

Fonte

PubMed

Palavras-Chave #Matrix-assisted Laser Desorption/ionization Time-of Flight Mass Spectrometry #Metabolic Fingerprinting #Plant Taxonomy #R Environment #Multivariate Data Analysis
Tipo

Artigo de periódico