Islet Neogenesis-associated Protein (ingap): The Role Of Its Endogenous Production As A Positive Modulator Of Insulin Secretion.
Contribuinte(s) |
UNIVERSIDADE DE ESTADUAL DE CAMPINAS |
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Data(s) |
27/11/2015
27/11/2015
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Resumo |
Islet neogenesis-associated protein (INGAP) is a peptide found in pancreatic exocrine-, duct- and islet- non-β-cells from normal hamsters. Its increase induced by either its exogenous administration or by the overexpression of its gene enhances β-cell secretory function and increases β-cell mass by a combination of stimulation of cell replication and islet neogenesis and reduction of β-cell apoptosis. We studied the potential modulatory role of endogenous INGAP in insulin secretion using two different experimental approaches. Hamster islets transfected with INGAP-small interfering RNA (INGAP-siRNA) were used to study glucose-stimulated insulin secretion (GSIS). In parallel, freshly isolated islets were incubated with high glucose and the same concentration of either a specific anti-INGAP rabbit serum or normal rabbit serum. INGAP-siRNA transfected islets reduced their INGAP mRNA and protein content by 35.1% and 47.2%, respectively whereas GSIS decreased by 25.8%. GSIS by transfected islets attained levels comparable to those recorded in control islets when INGAP pentadecapeptide (INGAP-PP) was added to the culture medium. INGAP antibody in the medium decreased significantly GSIS in a dose-dependent manner. These results indicate that endogenous INGAP plays a physiological positive modulatory role in insulin secretion, supporting its possible use in the treatment of prediabetes and Type 2 diabetes. 192-193 30-4 |
Identificador |
Regulatory Peptides. v. 192-193, p. 30-4 1873-1686 10.1016/j.regpep.2014.08.003 http://www.ncbi.nlm.nih.gov/pubmed/25160856 http://repositorio.unicamp.br/jspui/handle/REPOSIP/201664 25160856 |
Idioma(s) |
eng |
Relação |
Regulatory Peptides Regul. Pept. |
Direitos |
fechado Copyright © 2014 Elsevier B.V. All rights reserved. |
Fonte |
PubMed |
Palavras-Chave | #Hamsters #Ingap #Insulin Secretion #Isolated Islets #Sirna |
Tipo |
Artigo de periódico |