Islet Neogenesis-associated Protein (ingap): The Role Of Its Endogenous Production As A Positive Modulator Of Insulin Secretion.


Autoria(s): Flores, Luis E; Del Zotto, Héctor; Fragapane, Florencia; Maiztegui, Bárbara; Román, Carolina L; Boschero, Antonio C; Gagliardino, Juan J
Contribuinte(s)

UNIVERSIDADE DE ESTADUAL DE CAMPINAS

Data(s)

27/11/2015

27/11/2015

Resumo

Islet neogenesis-associated protein (INGAP) is a peptide found in pancreatic exocrine-, duct- and islet- non-β-cells from normal hamsters. Its increase induced by either its exogenous administration or by the overexpression of its gene enhances β-cell secretory function and increases β-cell mass by a combination of stimulation of cell replication and islet neogenesis and reduction of β-cell apoptosis. We studied the potential modulatory role of endogenous INGAP in insulin secretion using two different experimental approaches. Hamster islets transfected with INGAP-small interfering RNA (INGAP-siRNA) were used to study glucose-stimulated insulin secretion (GSIS). In parallel, freshly isolated islets were incubated with high glucose and the same concentration of either a specific anti-INGAP rabbit serum or normal rabbit serum. INGAP-siRNA transfected islets reduced their INGAP mRNA and protein content by 35.1% and 47.2%, respectively whereas GSIS decreased by 25.8%. GSIS by transfected islets attained levels comparable to those recorded in control islets when INGAP pentadecapeptide (INGAP-PP) was added to the culture medium. INGAP antibody in the medium decreased significantly GSIS in a dose-dependent manner. These results indicate that endogenous INGAP plays a physiological positive modulatory role in insulin secretion, supporting its possible use in the treatment of prediabetes and Type 2 diabetes.

192-193

30-4

Identificador

Regulatory Peptides. v. 192-193, p. 30-4

1873-1686

10.1016/j.regpep.2014.08.003

http://www.ncbi.nlm.nih.gov/pubmed/25160856

http://repositorio.unicamp.br/jspui/handle/REPOSIP/201664

25160856

Idioma(s)

eng

Relação

Regulatory Peptides

Regul. Pept.

Direitos

fechado

Copyright © 2014 Elsevier B.V. All rights reserved.

Fonte

PubMed

Palavras-Chave #Hamsters #Ingap #Insulin Secretion #Isolated Islets #Sirna
Tipo

Artigo de periódico