Identification Of Corynebacterium Spp. Isolated From Bovine Intramammary Infections By Matrix-assisted Laser Desorption Ionization-time Of Flight Mass Spectrometry.


Autoria(s): Gonçalves, Juliano Leonel; Tomazi, Tiago; Barreiro, Juliana Regina; Braga, Patrícia Aparecida de Campos; Ferreira, Christina Ramires; Araújo Junior, João Pessoa; Eberlin, Marcos Nogueira; dos Santos, Marcos Veiga
Contribuinte(s)

UNIVERSIDADE DE ESTADUAL DE CAMPINAS

Data(s)

01/09/2014

27/11/2015

27/11/2015

Resumo

Corynebacterium species (spp.) are among the most frequently isolated pathogens associated with subclinical mastitis in dairy cows. However, simple, fast, and reliable methods for the identification of species of the genus Corynebacterium are not currently available. This study aimed to evaluate the usefulness of matrix-assisted laser desorption ionization/mass spectrometry (MALDI-TOF MS) for identifying Corynebacterium spp. isolated from the mammary glands of dairy cows. Corynebacterium spp. were isolated from milk samples via microbiological culture (n=180) and were analyzed by MALDI-TOF MS and 16S rRNA gene sequencing. Using MALDI-TOF MS methodology, 161 Corynebacterium spp. isolates (89.4%) were correctly identified at the species level, whereas 12 isolates (6.7%) were identified at the genus level. Most isolates that were identified at the species level with 16 S rRNA gene sequencing were identified as Corynebacterium bovis (n=156; 86.7%) were also identified as C. bovis with MALDI-TOF MS. Five Corynebacterium spp. isolates (2.8%) were not correctly identified at the species level with MALDI-TOF MS and 2 isolates (1.1%) were considered unidentified because despite having MALDI-TOF MS scores >2, only the genus level was correctly identified. Therefore, MALDI-TOF MS could serve as an alternative method for species-level diagnoses of bovine intramammary infections caused by Corynebacterium spp.

173

147-51

Identificador

Veterinary Microbiology. v. 173, n. 1-2, p. 147-51, 2014-Sep.

1873-2542

10.1016/j.vetmic.2014.06.028

http://www.ncbi.nlm.nih.gov/pubmed/25086477

http://repositorio.unicamp.br/jspui/handle/REPOSIP/201603

25086477

Idioma(s)

eng

Relação

Veterinary Microbiology

Vet. Microbiol.

Direitos

fechado

Copyright © 2014 Elsevier B.V. All rights reserved.

Fonte

PubMed

Palavras-Chave #Animals #Cattle #Cattle Diseases #Corynebacterium #Corynebacterium Infections #Dairying #Female #Genes, Rrna #Mammary Glands, Animal #Milk #Rna, Ribosomal, 16s #Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization #Corynebacterium Spp. #Mass Spectrometry #Subclinical Mastitis
Tipo

Artigo de periódico