A RG-II type polysaccharide purified from Aconitum coreanum and their anti-inflammatory activity


Autoria(s): Li Xiaojun,; Jiang Jiaye,; Shi, S.S.; Li Yuan,; Jiang Yongbo,; Huang Dan Ke Yan,; Wang, S.C.; Bligh, S.W.A.; Ke, Yan
Data(s)

2014

Resumo

Korean mondshood root polysaccharides (KMPS) isolated from the root of Aconitum coreanum (Lévl.) Rapaics have shown anti-inflammatory activity, which is strongly influenced by their chemical structures and chain conformations. However, the mechanisms of the anti-inflammatory effect by these polysaccharides have yet to be elucidated. A RG-II polysaccharide (KMPS-2E, Mw 84.8 kDa) was isolated from KMPS and its chemical structure was characterized by FT-IR and NMR spectroscopy, gas chromatography–mass spectrometry and high-performance liquid chromatography. The backbone of KMPS-2E consisted of units of [→6) -β-D-Galp (1→3)-β-L-Rhap-(1→4)-β-D-GalpA-(1→3)-β-D-Galp-(1→] with the side chain →5)-β-D-Arap (1→3, 5)-β-D-Arap (1→ attached to the backbone through O-4 of (1→3,4)-L-Rhap. T-β-D-Galp is attached to the backbone through O-6 of (1→3,6)-β-D-Galp residues and T-β-D-Ara is connected to the end group of each chain. The anti-inflammatory effects of KMPS-2E and the underlying mechanisms using lipopolysaccharide (LPS) - stimulated RAW 264.7 macrophages and carrageenan-induced hind paw edema were investigated. KMPS-2E (50, 100 and 200 µg/mL) inhibits iNOS, TLR4, phospho-NF-κB–p65 expression, phosphor-IKK, phosphor-IκB-α expression as well as the degradation of IκB-α and the gene expression of inflammatory cytokines (TNF-α, IL-1β, iNOS and IL-6) mediated by the NF-κB signal pathways in macrophages. KMPS-2E also inhibited LPS-induced activation of NF-κB as assayed by electrophorectic mobility shift assay (EMSA) in a dose-dependent manner and it reduced NF-κB DNA binding affinity by 62.1% at 200µg/mL. In rats, KMPS-2E (200 mg/kg) can significantly inhibit carrageenan-induced paw edema as ibuprofen (200 mg/kg) within 3 h after a single oral dose. The results indicate that KMPS-2E is a promising herb-derived drug against acute inflammation.

Formato

application/pdf

Identificador

http://westminsterresearch.wmin.ac.uk/14039/1/Bligh_etal_PLoSOne_2014.pdf

Li Xiaojun, , Jiang Jiaye, , Shi, S.S., Li Yuan, , Jiang Yongbo, , Huang Dan Ke Yan, , Wang, S.C., Bligh, S.W.A. and Ke, Yan (2014) A RG-II type polysaccharide purified from Aconitum coreanum and their anti-inflammatory activity. PLoS ONE, 9 (6). e99697. ISSN 1932-6203

Idioma(s)

en

Publicador

Public Library of Science

Relação

http://westminsterresearch.wmin.ac.uk/14039/

https://dx.doi.org/10.1371/journal.pone.0099697

10.1371/journal.pone.0099697

Palavras-Chave #Science and Technology
Tipo

Article

PeerReviewed