Observation of an intact noncovalent homotrimer of detergent-solubilized rat microsomal glutathione transferase-1 by electrospray mass spectrometry


Autoria(s): Lengqvist, Johan; Svensson, Richard; Evergren, Emma; Morgenstern, Ralf; Griffiths, William J
Data(s)

02/04/2004

Resumo

<p>Microsomal glutathione transferase-1 (MGST1) is a membrane-bound enzyme involved in the detoxification of xenobiotics and the protection of cells against oxidative stress. The proposed active form of the enzyme is a noncovalently associated homotrimer that binds one substrate glutathione molecule/trimer. In this study, this complex has been directly observed by electrospray mass spectrometry analysis of active rat liver MGST1 reconstituted in a minimum amount of detergent. The measured mass of the homotrimer is 53 kDa, allowing for the mass of three MGST molecules in complex with one glutathione molecule. Collision-induced dissociation of the trimer complex resulted in the formation of monomer and homodimer ion species. Two distinct species of homodimer were observed, one unliganded and one identified as a homodimer.glutathione complex. Activation of the enzyme by N-ethylmaleimide through modification of Cys(49) (Svensson, R., Rinaldi, R., Swedmark, S., and Morgenstern, R. (2000) Biochemistry 39, 15144-15149) was monitored by the observation of an appropriate increase in mass in both the denatured monomeric and native trimeric forms of MGST1. Together, the data correspond well with the proposed functional organization of MGST1. These results also represent the first example of direct electrospray mass spectrometry analysis of a detergent-solubilized multimeric membrane protein complex in its native state.</p>

Identificador

http://pure.qub.ac.uk/portal/en/publications/observation-of-an-intact-noncovalent-homotrimer-of-detergentsolubilized-rat-microsomal-glutathione-transferase1-by-electrospray-mass-spectrometry(7f7aafbb-adb7-4cc7-a17c-3c173628f3ba).html

http://dx.doi.org/10.1074/jbc.M310958200

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Lengqvist , J , Svensson , R , Evergren , E , Morgenstern , R & Griffiths , W J 2004 , ' Observation of an intact noncovalent homotrimer of detergent-solubilized rat microsomal glutathione transferase-1 by electrospray mass spectrometry ' The Journal of biological chemistry , vol 279 , no. 14 , pp. 13311-6 . DOI: 10.1074/jbc.M310958200

Palavras-Chave #Animals #Detergents #Glutathione #Glutathione Transferase #Membrane Proteins #Microsomes #Octoxynol #Rats #Solubility #Spectrometry, Mass, Electrospray Ionization
Tipo

article