High frequency of donor chimerism after allogeneic transplantation of CD34+-selected peripheral blood cells


Autoria(s): Briones, J; Urbano-Ispizua, A; Lawler, M; Rozman, C; Gardiner, N; Marín, P; Salgado, C; Féliz, P; McCann, S; Montserrat, E; Lawler, Mark
Data(s)

01/05/1998

Resumo

<p>Ex vivo T cell depletion of allogeneic grafts is associated with a high (up to 80%) rate of mixed chimerism (MC) posttransplantation. The number of transplanted progenitor cells is an important factor in achieving complete donor chimerism in the T cell depletion setting. Use of granulocyte colony-stimulating factor (G-CSF) peripheral blood allografts allows the administration of large numbers of CD34+ cells. We studied the chimeric status of 13 patients who received allogeneic CD34+-selected peripheral blood progenitor cell transplants (allo-PBPCTs/CD34+) from HLA-identical sibling donors. Patients were conditioned with cyclophosphamide (120 mg/kg) and total-body irradiation (13 Gy in four fractions). Apheresis products were T cell-depleted by the immunoadsorption avidin-biotin method. The median number of CD34+ and CD3+ cells infused was 2.8x10(6)/kg (range 1.9-8.6x10(6)/kg) and 0.4x10(6)/kg (range 0.3-1x10(6)/kg), respectively. Molecular analysis of the engraftment was performed using polymerase chain reaction (PCR) amplification of highly polymorphic short tandem repeat (PCR-STR) sequences in peripheral blood samples. MC was detected in two (15%) of 13 patients. These two patients relapsed at 8 and 10 months after transplant, respectively. The remaining 11 patients showed complete donor chimerism and were in clinical remission after a maximum follow-up period of 24 months (range 6-24 months). These results were compared with those obtained in 10 patients who were treated with T cell-depleted bone marrow transplantation by means of elutriation and who received the same conditioning treatment and similar amounts of CD3+ cells (median 0.45x10(6)/kg; not significant) but a lower number of CD34+ cells (median 0.8x10(6)/kg; p = 0.001). MC was documented in six of 10 patients (60%), which was significantly higher than in the allo-PBPCT/CD34+ group (p = 0.04). We conclude that a high frequency of complete donor chimerism is achieved in patients receiving allo-PBPCT/CD34+ and that this is most likely due to the high number of progenitor cells administered.</p>

Identificador

http://pure.qub.ac.uk/portal/en/publications/high-frequency-of-donor-chimerism-after-allogeneic-transplantation-of-cd34selected-peripheral-blood-cells(cc39302d-b432-4aab-b0f3-b47ceefff07f).html

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Briones , J , Urbano-Ispizua , A , Lawler , M , Rozman , C , Gardiner , N , Marín , P , Salgado , C , Féliz , P , McCann , S , Montserrat , E & Lawler , M 1998 , ' High frequency of donor chimerism after allogeneic transplantation of CD34+-selected peripheral blood cells ' Experimental Hematology , vol 26 , no. 5 , pp. 415-20 .

Palavras-Chave #Adult #Antigens, CD34 #Bone Marrow Transplantation #Cytapheresis #Female #Graft vs Host Disease #Hematopoietic Stem Cell Transplantation #Hematopoietic Stem Cells #Humans #Incidence #Male #Middle Aged #Prospective Studies #Time Factors #Transplantation Chimera #Transplantation, Homologous
Tipo

article