Minimal residual disease detection in childhood acute lymphoblastic leukaemia patients at multiple time-points reveals high levels of concordance between molecular and immunophenotypic approaches


Autoria(s): Ryan, Jacqueline; Quinn, Fiona; Meunier, Armelle; Boublikova, Ludmila; Crampe, Mireille; Tewari, Prerna; O'Marcaigh, Aengus; Stallings, Ray; Neat, Michael; O'Meara, Ann; Breatnach, Fin; McCann, Shaun; Browne, Paul; Smith, Owen; Lawler, Mark; Lawler, Mark
Data(s)

01/01/2009

Resumo

<p>In this single centre study of childhood acute lymphoblastic leukaemia (ALL) patients treated on the Medical Research Council UKALL 97/99 protocols, it was determined that minimal residual disease (MRD) detected by real time quantitative polymerase chain reaction (RQ-PCR) and 3-colour flow cytometry (FC) displayed high levels of qualitative concordance when evaluated at multiple time-points during treatment (93.38%), and a combined use of both approaches allowed a multi time-point evaluation of MRD kinetics for 90% (53/59) of the initial cohort. At diagnosis, MRD markers with sensitivity of at least 0.01% were identified by RQ-PCR detection of fusion gene transcripts, IGH/TRG rearrangements, and FC. Using a combined RQ-PCR and FC approach, the evaluation of 367 follow-up BM samples revealed that the detection of MRD >1% at Day 15 (P = 0.04), >0.01% at the end of induction (P = 0.02), >0.01% at the end of consolidation (P = 0.01), >0.01% prior to the first delayed intensification (P = 0.01), and >0.1% prior to the second delayed intensification and continued maintenance (P = 0.001) were all associated with relapse and, based on early time-points (end of induction and consolidation) a significant log-rank trend (P = 0.0091) was noted between survival curves for patients stratified into high, intermediate and low-risk MRD groups.</p>

Identificador

http://pure.qub.ac.uk/portal/en/publications/minimal-residual-disease-detection-in-childhood-acute-lymphoblastic-leukaemia-patients-at-multiple-timepoints-reveals-high-levels-of-concordance-between-molecular-and-immunophenotypic-approaches(b9096832-dbda-47d0-b1e4-2a576219ebda).html

http://dx.doi.org/10.1111/j.1365-2141.2008.07429.x

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Ryan , J , Quinn , F , Meunier , A , Boublikova , L , Crampe , M , Tewari , P , O'Marcaigh , A , Stallings , R , Neat , M , O'Meara , A , Breatnach , F , McCann , S , Browne , P , Smith , O , Lawler , M & Lawler , M 2009 , ' Minimal residual disease detection in childhood acute lymphoblastic leukaemia patients at multiple time-points reveals high levels of concordance between molecular and immunophenotypic approaches ' British Journal of Haematology , vol 144 , no. 1 , pp. 107-15 . DOI: 10.1111/j.1365-2141.2008.07429.x

Palavras-Chave #Adolescent #Antineoplastic Combined Chemotherapy Protocols #Bone Marrow Examination #Child #Child, Preschool #Female #Flow Cytometry #Follow-Up Studies #Humans #Immunophenotyping #Infant #Kaplan-Meier Estimate #Male #Neoplasm, Residual #Precursor Cell Lymphoblastic Leukemia-Lymphoma #Prospective Studies #Reverse Transcriptase Polymerase Chain Reaction #Sensitivity and Specificity
Tipo

article