RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite


Autoria(s): McCoy, Ciaran J.; Warnock, Neil D.; Atkinson, Louise E.; Atcheson, Erwan; Martin, Richard J.; Robertson, Alan P.; Maule, Aaron G.; Marks, Nicola; Mousley, Angela
Data(s)

01/09/2015

Resumo

<p>The sustainable control of animal parasitic nematodes requires the development of efficient functional genomics platforms to facilitate target validation and enhance anthelmintic discovery. Unfortunately, the utility of RNA interference (RNAi) for the validation of novel drug targets in nematode parasites remains problematic. Ascaris suum is an important veterinary parasite and a zoonotic pathogen. Here we show that adult A. suum is RNAi competent, and highlight the induction, spread and consistency of RNAi across multiple tissue types. This platform provides a new opportunity to undertake whole organism-, tissue- and cell-level gene function studies to enhance target validation processes for nematode parasites of veterinary/medical significance.</p>

Formato

application/pdf

Identificador

http://pure.qub.ac.uk/portal/en/publications/rna-interference-in-adult-ascaris-suum--an-opportunity-for-the-development-of-a-functional-genomics-platform-that-supports-organism-tissue-and-cellbased-biology-in-a-nematode-parasite(ca17eee0-d063-4fad-982f-827073028000).html

http://dx.doi.org/10.1016/j.ijpara.2015.05.003

http://pure.qub.ac.uk/ws/files/16644581/Rna.pdf

Idioma(s)

eng

Direitos

info:eu-repo/semantics/openAccess

Fonte

McCoy , C J , Warnock , N D , Atkinson , L E , Atcheson , E , Martin , R J , Robertson , A P , Maule , A G , Marks , N & Mousley , A 2015 , ' RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite ' International Journal for Parasitology , vol 45 , no. 11 , pp. 673-678 . DOI: 10.1016/j.ijpara.2015.05.003

Palavras-Chave #Ascaris suum #Nematode parasite #Reverse genetics #RNA interference #/dk/atira/pure/subjectarea/asjc/2400/2405 #Parasitology #/dk/atira/pure/subjectarea/asjc/2700/2725 #Infectious Diseases
Tipo

article