Identification and Functional Analysis of a Novel Tryptophyllin Peptide from the Skin of the Red-eye Leaf Frog, Agalychnis callidryas


Autoria(s): Wang, Ran; Zhou, Yu; Chen, Tianbao; Zhou, Mei; Wang, Lei; Shaw, Chris
Data(s)

2015

Resumo

Amphibian skin has proved repeatedly to be a largely untapped source of bioactive peptides and this is especially true of members of the Phyllomedusinae subfamily of frogs native to South and Central America. Tryptophyllins are a group of peptides mainly found in the skin of members of this genus. In this study, a novel tryptophyllin (TPH) type 3 peptide, named AcT-3, has been isolated and structurally-characterised from the skin secretion and lyophilised skin extract of the red-eye leaf frog, Agalychnis callidryas. The peptide was identified in and purified from the skin secretion by reverse-phase HPLC. MALDI-TOF mass spectrometry and MS/MS fragmentation sequencing established its primary structure as: pGlu-Gly-Lys-Pro-Tyr-Trp-Pro-Pro-Pro-Phe-Leu-Pro-Glu, with a non-protonated molecular mass of 1538.19Da. The mature peptide possessed the canonical N-terminal pGlu residue that arises from post-translational modification of a Gln residue. The deduced open-reading frame consisted of 63 amino acid residues encoding a highly-conserved signal peptide of approximately 22 amino acid residues, an intervening acidic spacer peptide domain, a single AcT-3 encoding domain and a C terminal processing site. A synthetic replicate of AcT-3 was found to antagonise the effect of BK on rat tail artery smooth muscle and to contract the intestinal smooth muscle preparations. It was also found that AcT-3 could dose-dependently inhibit the proliferation of human prostate cancer cell lines after 72h incubation.

Formato

application/pdf

Identificador

http://pure.qub.ac.uk/portal/en/publications/identification-and-functional-analysis-of-a-novel-tryptophyllin-peptide-from-the-skin-of-the-redeye-leaf-frog-agalychnis-callidryas(c3644e74-bd17-4995-b9b9-4fec69515c63).html

http://dx.doi.org/10.7150/ijbs.10143

http://pure.qub.ac.uk/ws/files/17710394/v11p0209.pdf

Idioma(s)

eng

Direitos

info:eu-repo/semantics/openAccess

Fonte

Wang , R , Zhou , Y , Chen , T , Zhou , M , Wang , L & Shaw , C 2015 , ' Identification and Functional Analysis of a Novel Tryptophyllin Peptide from the Skin of the Red-eye Leaf Frog, Agalychnis callidryas ' International Journal of Biological Sciences , vol 11 , no. 2 , pp. 209-219 . DOI: 10.7150/ijbs.10143

Tipo

article