Enrichment of low-abundance proteins from bovine and porcine serum samples for proteomic studies


Autoria(s): Marco-Ramell, Anna; Bassols, Anna
Data(s)

01/12/2010

Resumo

One of the main applications of serum proteomics is the identification of new biomarkers for animal disease or animal production. However, potential obstacles to these studies are the poor performance of affinity serum depletion methods based on human antigens when using animal samples, and loss of minor serum components bound to albumin and other proteins. In the present study, we have analyzed the efficiency and reproducibility of the ProteoMiner® beads with bovine and porcine serum samples, and compared to a traditional immunoaffinity-based albumin and IgG depletion system specific for human samples. The ProteoMiner kit is based on the use of a combinatorial peptide binding library and intends to enrich low-abundance proteins.

Identificador

http://pure.qub.ac.uk/portal/en/publications/enrichment-of-lowabundance-proteins-from-bovine-and-porcine-serum-samples-for-proteomic-studies(2459717c-d023-4127-9786-e5b953b22712).html

http://dx.doi.org/10.1016/j.rvsc.2010.03.019

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Marco-Ramell , A & Bassols , A 2010 , ' Enrichment of low-abundance proteins from bovine and porcine serum samples for proteomic studies ' Research in Veterinary Science , vol 89 , no. 3 , pp. 340-343 . DOI: 10.1016/j.rvsc.2010.03.019

Tipo

article