Natural variation of rice strigolactone biosynthesis is associated with the deletion of two MAX1 orthologs
Data(s) |
11/02/2014
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Resumo |
Rice (Oryza sativa) cultivar Azucena--belonging to the Japonica subspecies--exudes high strigolactone (SL) levels and induces high germination of the root parasitic plant Striga hermonthica. Consistent with the fact that SLs also inhibit shoot branching, Azucena is a low-tillering variety. In contrast, Bala, an Indica cultivar, is a low-SL producer, stimulates less Striga germination, and is highly tillered. Using a Bala × Azucena F6 population, a major quantitative trait loci--qSLB1.1--for the exudation of SL, tillering, and induction of Striga germination was detected on chromosome 1. Sequence analysis of the corresponding locus revealed a rearrangement of a 51- to 59-kbp stretch between 28.9 and 29 Mbp in the Bala genome, resulting in the deletion of two cytochrome P450 genes--SLB1 and SLB2--with high homology to the Arabidopsis SL biosynthesis gene, MAX1. Both rice genes rescue the Arabidopsis max1-1 highly branched mutant phenotype and increase the production of the SL, ent-2'-epi-5-deoxystrigol, when overexpressed in Bala. Furthermore, analysis of this region in 367 cultivars of the publicly available Rice Diversity Panel population shows that the rearrangement at this locus is a recurrent natural trait associated with the Indica/Japonica divide in rice. |
Identificador | |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Cardoso , C , Zhang , Y , Jamil , M , Hepworth , J , Charnikhova , T , Dimkpa , S O N , Meharg , C , Wright , M H , Liu , J , Meng , X , Wang , Y , Li , J , McCouch , S R , Leyser , O , Price , A H , Bouwmeester , H J & Ruyter-Spira , C 2014 , ' Natural variation of rice strigolactone biosynthesis is associated with the deletion of two MAX1 orthologs ' Proceedings of the National Academy of Sciences of the United States of America , vol 111 , no. 6 , pp. 2379-2384 . DOI: 10.1073/pnas.1317360111 |
Palavras-Chave | #Chromosomes, Plant #Gene Deletion #Genes, Plant #Genetic Variation #Lactones #Molecular Sequence Data #Oryza sativa #Plant Proteins #Quantitative Trait Loci |
Tipo |
article |