In vitro o-antigen ligase assay
| Data(s) |
2013
|
|---|---|
| Resumo |
WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates. |
| Identificador | |
| Idioma(s) |
eng |
| Direitos |
info:eu-repo/semantics/restrictedAccess |
| Fonte |
Ruan , X & Valvano , M A 2013 , ' In vitro o-antigen ligase assay ' Methods in Molecular Biology , vol 1022 , pp. 185-97 . DOI: 10.1007/978-1-62703-465-4_15 |
| Tipo |
article |
