Alteration of beta-tubulin in Nicotiana plumbaginifolia confers resistance to amiprophos-methyl


Autoria(s): Blume, YB; Strashnyuk, NM; Smertenko, AP; Solodushko, VG; Sidorov, VA; Gleba, YY
Data(s)

01/08/1998

Resumo

<p>A Nicotiana plumbaginifolia plant (apm5(r)) resistant to amiprophos-methyl (APM), a phosphoroamide herbicide, was isolated from protoplasts prepared from leaves of haploid plants. Genetic analysis revealed that the resistance is coded for by a dominant nuclear mutation and is associated with the increased stability of cortical microtubules. Two-dimensional polyacrylamide-gel electrophoresis, combined with immunoblotting using anti-tubulin monoclonal antibodies, showed that part of the beta-tubulin in the resistant plant possessed lower isoelectric points than the beta-tubulin of susceptible wild-type plants. These results provide evidence that the resistance to APM is associated with a mutation in a beta-tubulin gene. The APM-resistant line showed cross-resistance to trifluralin, a dinitroaniline herbicide, suggesting a common mechanism of resistance between these two classes of herbicides.</p>

Identificador

http://pure.qub.ac.uk/portal/en/publications/alteration-of-betatubulin-in-nicotiana-plumbaginifolia-confers-resistance-to-amiprophosmethyl(4b059266-add4-4721-9369-653e7bb733fd).html

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Blume , Y B , Strashnyuk , N M , Smertenko , A P , Solodushko , V G , Sidorov , V A & Gleba , Y Y 1998 , ' Alteration of beta-tubulin in Nicotiana plumbaginifolia confers resistance to amiprophos-methyl ' Theoretical and Applied Genetics TAG , vol 97 , no. 3 , pp. 464-472 .

Palavras-Chave #CHLAMYDOMONAS #DINITROANILINE-RESISTANT #mutation #MICROTUBULE POLYMERIZATION INVITRO #CELLS #beta-tubulin #PLANT TUBULIN #amiprophos-methyl #CROSS-RESISTANCE #microtubules #PROTEINS #resistance #HERBICIDE #ORYZALIN #INHIBITION
Tipo

article