Local and systemic immune responses in mice to intranasal delivery of peptides representing bovine respiratory syncytial virus epitopes encapsulated in poly (dl-lactide-co-glycolide) microparticles


Autoria(s): Kavanagh, Owen V; Adair, Brian M; Welsh, Michael D; Earley, Bernadette
Data(s)

01/06/2013

Resumo

The potential of a microparticulate vaccine delivery system in eliciting a specific mucosal antibody response in the respiratory tract of mice was evaluated. Two vaccine candidate peptides representing epitopes from the G attachment and F fusion antigens from bovine respiratory syncytial virus (BRSV) were encapsulated into poly(dl- lactide co-glycolide) biodegradable microparticles. The encapsulation process did not denature the entrapped peptides as verified by detection of peptide-specific antibodies in mucosal secretions by ELISA using peptide as antigen. Following intranasal immunisation, the encapsulated peptides induced stronger upper and lower respiratory tract specific-IgA responses, respectively, than the soluble peptide forms. Moreover, a strong peptide-specific cell-mediated immune response was measured in splenocytes in vitro from the mice inoculated with the encapsulated peptides compared to their soluble form alone indicating that migration of primed T cells had taken place from the site of mucosal stimulation in the upper respiratory tract to the spleen. These results act as a foundation for vaccine efficacy studies in large animal BRSV challenge models.

Identificador

http://pure.qub.ac.uk/portal/en/publications/local-and-systemic-immune-responses-in-mice-to-intranasal-delivery-of-peptides-representing-bovine-respiratory-syncytial-virus-epitopes-encapsulated-in-poly-dllactidecoglycolide-microparticles(9558d31b-647d-4836-bcb3-4ea1a3753ff3).html

http://dx.doi.org/10.1016/j.rvsc.2012.12.001

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Kavanagh , O V , Adair , B M , Welsh , M D & Earley , B 2013 , ' Local and systemic immune responses in mice to intranasal delivery of peptides representing bovine respiratory syncytial virus epitopes encapsulated in poly (dl-lactide-co-glycolide) microparticles ' Research in Veterinary Science , vol 94 , no. 3 , pp. 809-12 . DOI: 10.1016/j.rvsc.2012.12.001

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/3400 #veterinary(all)
Tipo

article