Role of Bacterial Surface Structures on the Interaction of <i>Klebsiella pneumoniae</i> with Phagocytes


Autoria(s): March, Catalina; Cano, Victoria; Moranta, David; Llobet, Enrique; Pérez-Gutiérrez, Camino; Tomás, Juan M.; Suárez, Teresa; Garmendia, Junkal; Bengoechea, José A.
Data(s)

15/02/2013

Resumo

Phagocytosis is a key process of the immune system. The human pathogen Klebsiella pneumoniae is a well known example of a pathogen highly resistant to phagocytosis. A wealth of evidence demonstrates that the capsule polysaccharide (CPS) plays a crucial role in resistance to phagocytosis. The amoeba Dictyostelium discoideum shares with mammalian macrophages the ability to phagocytose and kill bacteria. The fact that K. pneumoniae is ubiquitous in nature and, therefore, should avoid predation by amoebae, poses the question whether K. pneumoniae employs similar means to counteract amoebae and mammalian phagocytes. Here we developed an assay to evaluate K. pneumoniae-D. discoideum interaction. The richness of the growth medium affected the threshold at which the cps mutant was permissive for Dictyostelium and only at lower nutrient concentrations the cps mutant was susceptible to predation by amoebae. Given the critical role of bacterial surface elements on host-pathogen interactions, we explored the possible contribution of the lipopolysaccharide (LPS) and outer membrane proteins (OMPs) to combat phagoyctosis by D. discoideum. We uncover that, in addition to the CPS, the LPS O-polysaccharide and the first core sugar participate in Klebsiella resistance to predation by D. discoideum. K. pneumoniae LPS lipid A decorations are also necessary to avoid predation by amoebae although PagP-dependent palmitoylation plays a more important role than the lipid A modification with aminoarabinose. Mutants lacking OMPs OmpA or OmpK36 were also permissive for D. discoideium growth. Except the LPS O-polysaccharide mutants, all mutants were more susceptible to phagocytosis by mouse alveolar macrophages. Finally, we found a correlation between virulence, using the pneumonia mouse model, and resistance to phagocytosis. Altogether, this work reveals novel K. pneumoniae determinants involved in resistance to phagocytosis and supports the notion that Dictyostelium amoebae might be useful as host model to measure K. pneumoniae virulence and not only phagocytosis. © 2013 March et al.

Formato

application/pdf

Identificador

http://pure.qub.ac.uk/portal/en/publications/role-of-bacterial-surface-structures-on-the-interaction-of-klebsiella-pneumoniae-with-phagocytes(64c409a5-c879-49ea-97d2-230adafd9b47).html

http://dx.doi.org/10.1371/journal.pone.0056847

http://pure.qub.ac.uk/ws/files/15851119/role_of_bacterial_surface_structures.pdf

http://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-84874066976&md5=1f45ecf3991e3aaf5f27e773d501b441

Idioma(s)

eng

Direitos

info:eu-repo/semantics/openAccess

Fonte

March , C , Cano , V , Moranta , D , Llobet , E , Pérez-Gutiérrez , C , Tomás , J M , Suárez , T , Garmendia , J & Bengoechea , J A 2013 , ' Role of Bacterial Surface Structures on the Interaction of Klebsiella pneumoniae with Phagocytes ' PLoS One , vol 8 , no. 2 , e56847 . DOI: 10.1371/journal.pone.0056847

Tipo

article