A tripartite microbial reporter gene system for real-time assays of soil nutrient status
Data(s) |
14/03/2003
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Resumo |
Plant-derived carbon is the substrate which drives the rate of microbial assimilation and turnover of nutrients, in particular N and P, within the rhizosphere. To develop a better understanding of rhizosphere dynamics, a tripartite reporter gene system has been developed. We used three lux-marked Pseudomonas fluorescens strains to report on soil (1) assimilable carbon, (2) N-status, and (3) P-status. In vivo studies using soil water, spiked with C, N and P to simulate rhizosphere conditions, showed that the tripartite reporter system can provide real-time assessment of carbon and nutrient status. Good quantitative agreement for bioluminescence output between reference material and soil water samples was found for the C and P reporters. With regard to soil nitrate, the minimum bioavailable concentration was found to be greater than that analytically detectable in soil water. This is the first time that bioavailable soil C, N and P have been quantified using a tripartite reporter gene system. |
Identificador | |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Standing , D , Meharg , A A , Killham , K & Meharg , A 2003 , ' A tripartite microbial reporter gene system for real-time assays of soil nutrient status ' FEMS Microbiology Letters , vol 220 , no. 1 , pp. 35-9 . |
Palavras-Chave | #/dk/atira/pure/subjectarea/asjc/1300/1311 #Genetics #/dk/atira/pure/subjectarea/asjc/1300/1312 #Molecular Biology #/dk/atira/pure/subjectarea/asjc/2400/2402 #Applied Microbiology and Biotechnology #/dk/atira/pure/subjectarea/asjc/2400/2404 #Microbiology |
Tipo |
article |