Plume characteristics and dynamics of UV and IR laser-desorbed oligonucleotides


Autoria(s): Merrigan, Tony L; Timson, David J; Hunniford, C. Adam; Catney, Martin; McCullough, Robert W
Data(s)

01/05/2012

Resumo

Laser desorption of dye-tagged oligonucleotides was studied using laser-induced fluorescence imaging. Desorption with ultra violet (UV) and infra-red (IR) lasers resulted in forward directed plumes of molecules. In the case of UV desorption, the initial shot desorbed approximately seven-fold more material than subsequent shots. In contrast, the initial shot in IR desorption resulted in the ejection of less material compared to subsequent shots and these plumes had a component directed along the path of the laser. Thermal equilibrium of the molecules in the plume was achieved after approximately 25 µs with a spread in molecular temperature which was described by a modified Maxwell-Boltzmann equation.

Identificador

http://pure.qub.ac.uk/portal/en/publications/plume-characteristics-and-dynamics-of-uv-and-ir-laserdesorbed-oligonucleotides(b41d34d9-dc9c-4891-bacb-574d8952720f).html

http://dx.doi.org/10.1016/j.ijbiomac.2012.03.007

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Merrigan , T L , Timson , D J , Hunniford , C A , Catney , M & McCullough , R W 2012 , ' Plume characteristics and dynamics of UV and IR laser-desorbed oligonucleotides ' International journal of biological macromolecules , vol 50 , no. 4 , pp. 1081-90 . DOI: 10.1016/j.ijbiomac.2012.03.007

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/1300/1303 #Biochemistry #/dk/atira/pure/subjectarea/asjc/1300/1312 #Molecular Biology #/dk/atira/pure/subjectarea/asjc/1300/1315 #Structural Biology
Tipo

article