Organization of mammary epithelial cells into 3D acinar structures requires glucocorticoid and JNK signaling
Data(s) |
05/07/2004
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Resumo |
Mammary epithelial cells cultured on a concentrated laminin-rich extracellular matrix formed 3D acinar structures that matured to polarized monolayers surrounding a lumen. In the absence of glucocorticoids mature acinus formation failed and the expression of an acinus-associated, activator protein 1 (AP1) and nuclear factor kappaB transcription factor DNA-binding profile was lost. Treatment with the JNK inhibitor, SP600125, caused similar effects, whereas normal organization of the mammary epithelial cells as acini caused JNK activation in a glucocorticoid-dependent manner. The forming acini expressed BRCA1, GADD45beta, MEKK4, and the JNK activating complex GADD 45beta-MEKK4 in a glucocorticoid-dependent fashion. JNK catalyzed phosphorylation of c-Jun was also detected in the acini. In addition, expression of beta4 integrin and in situ occupation of its promoter by AP1 components, c-Jun and Fos, was glucocorticoid dependent. These results suggest that glucocortocoid signaling regulates acinar integrity through a pathway involving JNK regulation of AP1 transcription factors and beta4 integrin expression. |
Identificador | |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Murtagh , J , McArdle , E , Gilligan , E , Thornton , L , Furlong , F & Martin , F 2004 , ' Organization of mammary epithelial cells into 3D acinar structures requires glucocorticoid and JNK signaling ' The Journal of cell biology , vol 166 , no. 1 , pp. 133-43 . DOI: 10.1083/jcb.200403020 |
Palavras-Chave | #/dk/atira/pure/subjectarea/asjc/1300/1307 #Cell Biology |
Tipo |
article |