An amino acid substitution in Fasciola hepatica P-glycoprotein from triclabendazole-resistant and triclabendazole-susceptible populations


Autoria(s): Wilkinson, R.; Law, C.J.; Hoey, E.M.; Fairweather, I.; Brennan, G.P.; Trudgett, A.
Data(s)

01/11/2012

Resumo

Control of fasciolosis is threatened by the development of anthelmintic resistance. Enhanced triclabendazole (TCBZ) efflux by ABC transporters such as P-glycoprotein (Pgp) has been implicated in this process. A putative full length cDNA coding for a Pgp expressed in adult Fasciola hepatica has been constructed and used to design a primer set capable of amplifying a region encoding part of the second nucleotide binding domain of Pgp when genomic DNA was used as a template. Application of this primer set to genomic DNA from TCBZ-resistant and -susceptible field populations has shown a significant difference in the alleles present. Analysis of an allele occurring at a three-fold higher frequency in the "resistant" population revealed that it was characterised by a serine to arginine substitution at residue 1144. Homology modelling studies have been used to locate this site in the Pgp structure and hence assess its potential to modify functional activity. © 2012 Elsevier B.V.

Formato

application/pdf

Identificador

http://pure.qub.ac.uk/portal/en/publications/an-amino-acid-substitution-in-fasciola-hepatica-pglycoprotein-from-triclabendazoleresistant-and-triclabendazolesusceptible-populations(60c602d4-b7f2-4901-a163-b600c8f51ff0).html

http://dx.doi.org/10.1016/j.molbiopara.2012.08.008

http://pure.qub.ac.uk/ws/files/2395607/An_amino_acid_substitution_in_Fasciola_hepatica_P_glycoprotein_from_triclabendazole_resistant_and_triclabendazole_susceptible_populations.pdf

Idioma(s)

eng

Direitos

info:eu-repo/semantics/openAccess

Fonte

Wilkinson , R , Law , C J , Hoey , E M , Fairweather , I , Brennan , G P & Trudgett , A 2012 , ' An amino acid substitution in Fasciola hepatica P-glycoprotein from triclabendazole-resistant and triclabendazole-susceptible populations ' Molecular and Biochemical Parasitology , vol 186 , no. 1 , pp. 69-72 . DOI: 10.1016/j.molbiopara.2012.08.008

Palavras-Chave #Fasciola hepatica; Anthelmintic resistance; P-glycoprotein; SNP #/dk/atira/pure/subjectarea/asjc/2400/2405 #Parasitology #/dk/atira/pure/subjectarea/asjc/1300/1312 #Molecular Biology
Tipo

article