Influence of alpha-helicity, amphipathicity and D-amino acid incorporation on the peptide-induced mast cell activation


Autoria(s): Cross, L.J.M.; Ennis, Madeleine; Krause, E.; Dathe, M.; Lorenz, D.; Krause, G.; Beyermann, M.; Bienert, M.
Data(s)

1995

Resumo

Mast cell activation by polycationic substances is believed to result from a direct activation of G protein alpha subunits and it was suggested that the adaption of amphipathic, alpha-helical conformations would allow the peptide to reach the cytosolic compartment to interact with G proteins (Mousli et al., 1994, Immunopharmacology 27, 1, for review). We investigated the histamine-releasing activity of model peptides as well as analogues of magainin 2 amide and neuropeptide Y with different amphipathicities and alpha-helix content on rat peritoneal mast cells. Amphipathic helicity is not a prerequisite for mast cell activation. Moreover, non-helical magainin peptides with high histamine-releasing activity were less active in the liberation of carboxyfluoresceine from negatively charged liposomes, indicating that peptide-induced mast cell activation and peptide-induced membrane perturbation do not correlate. In contrast to the negligible influence of the secondary structure, amino acid configuration may exert a striking influence on peptide-induced mast cell activation. Thus histamine-release by substance P was markedly impaired when the L-amino acids in the positively charged N-terminal region were replaced by D-amino acids, with [D-Arg(1)]substance P being the most inactive substance P diastereoisomer.

Identificador

http://pure.qub.ac.uk/portal/en/publications/influence-of-alphahelicity-amphipathicity-and-damino-acid-incorporation-on-the-peptideinduced-mast-cell-activation(29bfffda-b2da-4bee-a054-8c7c03c4f417).html

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Cross , L J M , Ennis , M , Krause , E , Dathe , M , Lorenz , D , Krause , G , Beyermann , M & Bienert , M 1995 , ' Influence of alpha-helicity, amphipathicity and D-amino acid incorporation on the peptide-induced mast cell activation ' EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION , vol 291 , pp. 291-300 .

Tipo

article