The effect of triclabendazole ("Fasinex") on protein synthesis by the liver fluke, Fasciola hepatica


Autoria(s): Stitt, Alan; Fairweather, Ian; Mackender, R.O.
Data(s)

1995

Resumo

The effect of the active sulphoxide metabolite of the anthelmintic triclabendazole (TCBZ-SX, 15-50 mu g ml(-1)) on the incorporation of radioactively labelled [C-14] leucine by adult Fasciola hepatica tissue slices was measured by liquid scintillation counting. In addition, the ability of the microfilament-disrupting drug, cytochalasin B, and the microtubule-disrupting drug, tubulozole-C, to inhibit protein synthesis, was assessed by similar methods and compared with TCBZ-SX. The established protein synthesis inhibitors, cycloheximide and actinomycin D were used as positive controls. All the drugs showed a significant inhibition of protein synthesis, albeit to different extents; however, TCBZ-SX was the most potent, with no significant difference between its effect and that of cycloheximide or actinomycin D. Moreover, the concentration of TCBZ-SX, above 15 mu g ml(-1), had little further influence on incorporation of [C-14] leucine. This investigation demonstrates the inhibitory effect of TCBZ-SX, cytochalasin B and tubulozole-C on protein synthesis in F. hepatica and confirms the qualitative observations made in several previous ultrastructural studies.

Identificador

http://pure.qub.ac.uk/portal/en/publications/the-effect-of-triclabendazole-fasinex-on-protein-synthesis-by-the-liver-fluke-fasciola-hepatica(57b68681-220a-4f28-8450-2db60564375b).html

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Stitt , A , Fairweather , I & Mackender , R O 1995 , ' The effect of triclabendazole ("Fasinex") on protein synthesis by the liver fluke, Fasciola hepatica ' International Journal for Parasitology , vol 25 , pp. 421-429 .

Tipo

article