AFP(+), ESC-derived cells engraft and differentiate into hepatocytes in vivo


Autoria(s): Yin, Y.; Lim, Y.K.; Salto-Tellez, Manuel; Ng, S.C.; Lin, C.S.; Lim, S.K.
Data(s)

2002

Resumo

A major problem in gene therapy and tissue replacement is accessibility of tissue-specific stem cells. One solution is to isolate tissue-specific stem cells from differentiating embryonic stem (ES) cells. Here, we show that liver progenitor cells can be purified from differentiated ES cells using alpha-fetoprotein (AFP) as a marker. By knocking the green fluorescent protein (GFP) gene into the AFP locus of ES cells and differentiating the modified ES cells in vitro, a subpopulation of GFP(+) and AFP-expressing cells was generated. When transplanted into partially hepatectomized lacZ-positive ROSA26 mice, GFP(+) cells engrafted and differentiated into lacZ-negative and albumin-positive hepatocytes. Differentiation into hepatocytes also occurred after transplantation of GFP(+) cells in apolipoprotein-E- (ApoE) or haptoglobin-deficient mice as demonstrated by the presence of ApoE-positive hepatocytes and ApoE mRNA in the liver of ApoE-deficient mice or by haptoglobin in the serum and haptoglobin mRNA in the liver of haptoglobin-deficient mice. This study describes the first isolation of ES-cell-derived liver progenitor cells that are viable mediators of liver-specific functions in vivo.

Identificador

http://pure.qub.ac.uk/portal/en/publications/afp-escderived-cells-engraft-and-differentiate-into-hepatocytes-in-vivo(9cb50bba-b728-4140-ab05-5cd975174691).html

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Yin , Y , Lim , Y K , Salto-Tellez , M , Ng , S C , Lin , C S & Lim , S K 2002 , ' AFP(+), ESC-derived cells engraft and differentiate into hepatocytes in vivo ' Stem Cells , vol 20 , no. 4 , pp. 338-346 .

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/1300/1307 #Cell Biology
Tipo

article