Multiplex RT-PCR for the detection of leukemia-associated translocations - Validation and application to routine molecular diagnostic practice


Autoria(s): Salto-Tellez, Manuel; Shelat, S.G.; Benoit, B.; Rennert, H.; Carroll, M.; Leonard, D.G.B.; Nowell, P.; Bagg, A.
Data(s)

01/11/2003

Resumo

The aim of this study was to validate the application of a commercially available multiplex reverse transcription polymerase chain reaction (RT-PCR) assay [He-mavision-7 System] for the seven most common leukemia translocations for routine molecular diagnostic hematopathology practice. A total of 98 samples, comprising four groups, were evaluated: Group 1, 16 diagnostic samples molecularly positive by our existing laboratory-developed assays for PML-RARalpha/t (15; 17) or BCR-ABL/t (9;22); Group 2, 51 diagnostic samples negative by our laboratory-developed assays for PML-RARalpha/t (15;17) or BCR-ABL/t (9;22); Group 3, 21 prospectively analyzed diagnostic cases, without prior molecular studies; and Group 4, 10 minimal residual disease (MRD) samples. Analysis of the two previously studied cohorts (Groups 1 and 2) confirmed the diagnostic sensitivity and specificity of the multiplex assay with regard to these two translocations. Additionally, however, in the

Identificador

http://pure.qub.ac.uk/portal/en/publications/multiplex-rtpcr-for-the-detection-of-leukemiaassociated-translocations--validation-and-application-to-routine-molecular-diagnostic-practice(2d98c790-5b84-4678-acfb-93a0e4de997a).html

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Salto-Tellez , M , Shelat , S G , Benoit , B , Rennert , H , Carroll , M , Leonard , D G B , Nowell , P & Bagg , A 2003 , ' Multiplex RT-PCR for the detection of leukemia-associated translocations - Validation and application to routine molecular diagnostic practice ' Journal of Molecular Diagnostics , vol 5 , no. 4 , pp. 231-236 .

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/1300/1312 #Molecular Biology
Tipo

article