Chronic treatment with a stable obestatin analogue significantly alters plasma triglyceride levels but fails to influence food intake, fluid intake, body weight, or body composition in rats.


Autoria(s): Agnew, A.; Calderwood, D.; Chevallier, O.P.; Greer, B.; Grieve, David; Green, B.D.
Data(s)

01/03/2011

Resumo

Obestatin (OB(1-23) is a 23 amino acid peptide encoded on the preproghrelin gene, originally reported to have metabolic actions related to food intake, gastric emptying and body weight. The biological instability of OB(1-23) has recently been highlighted by studies demonstrating its rapid enzymatic cleavage in a number of biological matrices. We assessed the stability of both OB(1-23) and an N-terminally PEGylated analogue (PEG-OB(1-23)) before conducting chronic in vivo studies. Peptides were incubated in rat liver homogenate and degradation monitored by LC-MS. PEG-OB(1-23) was approximately 3-times more stable than OB(1-23). Following a 14 day infusion of Sprague Dawley rats with 50 mol/kg/day of OB(1-23) or a N-terminally PEGylated analogue (PEG-OB(1-23)), we found no changes in food/fluid intake, body weight and plasma glucose or cholesterol between groups. Furthermore, morphometric liver, muscle and white adipose tissue (WAT) weights and tissue triglyceride concentrations remained unaltered between groups. However, with stabilised PEG-OB(1-23) we observed a 40% reduction in plasma triglycerides. These findings indicate that PEG-OB(1-23) is an OB(1-23) analogue with significantly enhanced stability and suggest that obestatin could play a role in modulating physiological lipid metabolism, although it does not appear to be involved in regulation of food/fluid intake, body weight or fat deposition.

Formato

application/pdf

Identificador

http://pure.qub.ac.uk/portal/en/publications/chronic-treatment-with-a-stable-obestatin-analogue-significantly-alters-plasma-triglyceride-levels-but-fails-to-influence-food-intake-fluid-intake-body-weight-or-body-composition-in-rats(69a05c24-b5c4-40dc-9e8b-434b88725f4d).html

http://dx.doi.org/10.1016/j.peptides.2010.12.005

http://pure.qub.ac.uk/ws/files/790451/Agnew%20et%20al%20Peptides%202011.pdf

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Agnew , A , Calderwood , D , Chevallier , O P , Greer , B , Grieve , D & Green , B D 2011 , ' Chronic treatment with a stable obestatin analogue significantly alters plasma triglyceride levels but fails to influence food intake, fluid intake, body weight, or body composition in rats. ' Peptides , vol 32 , no. 4 , pp. 755-762 . DOI: 10.1016/j.peptides.2010.12.005

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/1300/1303 #Biochemistry #/dk/atira/pure/subjectarea/asjc/1300/1310 #Endocrinology #/dk/atira/pure/subjectarea/asjc/1300/1314 #Physiology #/dk/atira/pure/subjectarea/asjc/2800/2804 #Cellular and Molecular Neuroscience
Tipo

article