Ribose 2'-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5.
Data(s) |
01/01/2011
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Resumo |
The 5' cap structures of higher eukaryote mRNAs have ribose 2'-O-methylation. Likewise, many viruses that replicate in the cytoplasm of eukaryotes have evolved 2'-O-methyltransferases to autonomously modify their mRNAs. However, a defined biological role for 2'-O-methylation of mRNA remains elusive. Here we show that 2'-O-methylation of viral mRNA was critically involved in subverting the induction of type I interferon. We demonstrate that human and mouse coronavirus mutants lacking 2'-O-methyltransferase activity induced higher expression of type I interferon and were highly sensitive to type I interferon. Notably, the induction of type I interferon by viruses deficient in 2'-O-methyltransferase was dependent on the cytoplasmic RNA sensor Mda5. This link between Mda5-mediated sensing of viral RNA and 2'-O-methylation of mRNA suggests that RNA modifications such as 2'-O-methylation provide a molecular signature for the discrimination of self and non-self mRNA. |
Identificador | |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Zust , R , Cervantes-Barragan , L , Habjan , M , Maier , R , Neuman , B W , Ziebuhr , J , Szretter , K J , Baker , S C , Barchet , W , Diamond , M S , Siddell , S G , Ludewig , B & Thiel , V 2011 , ' Ribose 2'-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5. ' Nature Immunology , vol 12(2) , no. 2 , pp. 137-143 . DOI: 10.1038/ni.1979 |
Palavras-Chave | #/dk/atira/pure/subjectarea/asjc/2400/2403 #Immunology |
Tipo |
article |