Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation
Data(s) |
2000
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Resumo |
We irradiated different cellular compartments and measured changes in expression of the FOS gene at the mRNA and protein levels. [H-3]Thymidine and tritiated water were used to irradiate the nucleus and the whole cell, respectively. I-125-Concanavalin A binding was used to irradiate the cell membrane differentially. Changes in FOS mRNA and protein levels were measured using semi-quantitative RT-PCR and SDS-PAGE Western blotting, respectively, Irradiation of the nucleus or the whole cell at a dose rate of 0.075 Gy/h caused no change in the level of FOS mRNA expression, but modestly (1.5-fold) induced FOS protein after 0.5 h, Irradiation of the nucleus at a dose rate of 0.43 Gy/h induced FOS mRNA by 1.5-fold after 0.5 h, but there was no significant effect after whole-cell irradiation. FOS protein was transiently induced 2.5-fold above control levels 0.5 h after a 0.43-Gy/h exposure of the nucleus or the whole cell. Irradiation of the cell membrane at a dose rate of 1.8 Gy/h for up to 2 h caused no change in the levels of expression of FOS mRNA or protein, but a dose rate of 6.8 Gy/h transiently increased the level of FOS mRNA S-fold after 0.5 h, These data demonstrate the complexity of the cellular response to radiation-induced damage at low doses. The lack of quantitative agreement between the transcript and protein levels for FOS suggests a role for posttranscriptional regulation. (C) 2000 by Radiation Research Society. |
Identificador |
http://www.scopus.com/inward/record.url?scp=0033778035&partnerID=8YFLogxK |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Al-Assar , O , Robson , T , McKeown , S R , Gardin , I , Wilson , G D & Hirst , D 2000 , ' Regulation of FOS by different compartmental stresses induced by low levels of ionizing radiation ' Radiation Research , vol 154 , no. 5 , pp. 503-514 . |
Palavras-Chave | #/dk/atira/pure/subjectarea/asjc/1100/1101 #Agricultural and Biological Sciences (miscellaneous) #/dk/atira/pure/subjectarea/asjc/1300/1304 #Biophysics #/dk/atira/pure/subjectarea/asjc/2700/2741 #Radiology Nuclear Medicine and imaging #/dk/atira/pure/subjectarea/asjc/3100/3108 #Radiation |
Tipo |
article |