PAR-2 Regulates Dental Pulp Inflammation Associated with Caries


Autoria(s): Lundy, Fionnuala; About, I.; Curtis, Timothy; McGahon, Mary; Linden, Gerard; Irwin, Christopher; El Karim, Ikhlas
Data(s)

01/07/2010

Resumo

Protease-activated receptors (PARs) are G-protein-coupled receptors that are activated enzymatically by proteolysis of an N-terminal domain. The cleavage and activation of PARs by serine proteases represent a novel mechanism by which such enzymes could influence the host inflammatory response. The aim of this study was to determine whether PAR-2 expression and activation were increased in dental caries. Using immunohistochemistry, we showed PAR-2 to be localized to pulp cells subjacent to caries lesions, but minimally expressed by healthy pulp tissue. Trypsin and the PAR-2 agonist (PAR2-AP) activated PAR-2 in an in vitro functional assay. Endogenous molecules present in pulp cell lysates from carious teeth specifically activated PAR-2, but those from healthy teeth failed to do so. The activation of PAR-2 in vitro was shown to increase the expression of the pro-inflammatory mediator cyclo-oxygenase-2 (COX-2), providing a mechanism whereby PAR-2 could modulate pulpal inflammation.

Identificador

http://pure.qub.ac.uk/portal/en/publications/par2-regulates-dental-pulp-inflammation-associated-with-caries(479bd6e7-a914-4780-a713-684f3b7dff3d).html

http://dx.doi.org/10.1177/0022034510365652

http://www.scopus.com/inward/record.url?scp=77953772578&partnerID=8YFLogxK

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Lundy , F , About , I , Curtis , T , McGahon , M , Linden , G , Irwin , C & El Karim , I 2010 , ' PAR-2 Regulates Dental Pulp Inflammation Associated with Caries ' Journal of Dental Research , vol 89 , no. 7 , pp. 684-688 . DOI: 10.1177/0022034510365652

Palavras-Chave #dental pulp #human #inflammation #PAR #/dk/atira/pure/subjectarea/asjc/3500 #Dentistry(all)
Tipo

article