PAR-2 Regulates Dental Pulp Inflammation Associated with Caries
Data(s) |
01/07/2010
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Resumo |
Protease-activated receptors (PARs) are G-protein-coupled receptors that are activated enzymatically by proteolysis of an N-terminal domain. The cleavage and activation of PARs by serine proteases represent a novel mechanism by which such enzymes could influence the host inflammatory response. The aim of this study was to determine whether PAR-2 expression and activation were increased in dental caries. Using immunohistochemistry, we showed PAR-2 to be localized to pulp cells subjacent to caries lesions, but minimally expressed by healthy pulp tissue. Trypsin and the PAR-2 agonist (PAR2-AP) activated PAR-2 in an in vitro functional assay. Endogenous molecules present in pulp cell lysates from carious teeth specifically activated PAR-2, but those from healthy teeth failed to do so. The activation of PAR-2 in vitro was shown to increase the expression of the pro-inflammatory mediator cyclo-oxygenase-2 (COX-2), providing a mechanism whereby PAR-2 could modulate pulpal inflammation. |
Identificador |
http://dx.doi.org/10.1177/0022034510365652 http://www.scopus.com/inward/record.url?scp=77953772578&partnerID=8YFLogxK |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Lundy , F , About , I , Curtis , T , McGahon , M , Linden , G , Irwin , C & El Karim , I 2010 , ' PAR-2 Regulates Dental Pulp Inflammation Associated with Caries ' Journal of Dental Research , vol 89 , no. 7 , pp. 684-688 . DOI: 10.1177/0022034510365652 |
Palavras-Chave | #dental pulp #human #inflammation #PAR #/dk/atira/pure/subjectarea/asjc/3500 #Dentistry(all) |
Tipo |
article |