Priming of CD8+ and CD4+ T Cells in Experimental Leishmaniasis Is Initiated by Different Dendritic Cell Subtypes


Autoria(s): Brewig, Nancy; Kissenpfennig, Adrien; Malissen, Bernard; Veit, Alexandra; Bickert, Thomas; Fleischer, Bernhard; Mostböck, Sven; Ritter, Uwe
Data(s)

15/01/2009

Resumo

The biological role of Langerin(+) dendritic cells (DCs) such as Langerhans cells and a subset of dermal DCs (dDCs) in adaptive immunity against cutaneous pathogens remains enigmatic. Thus, we analyzed the impact of Langerin(+) DCs in adaptive T cell-mediated immunity toward Leishmania major parasites in a Lang-DTR mouse model that allows conditional diphtheria toxin (DT)-induced ablation of The biological role of Langerin+ dendritic cells (DCs) such as Langerhans cells and a subset of dermal DCs (dDCs) in adaptive immunity against cutaneous pathogens remains enigmatic. Thus, we analyzed the impact of Langerin+ DCs in adaptive T cell-mediated immunity toward Leishmania major parasites in a Lang-DTR mouse model that allows conditional diphtheria toxin (DT)-induced ablation of Langerin+ DCs in vivo. For the first time, infection experiments with DT-treated Lang-DTR mice revealed that proliferation of L. major-specific CD8+ T cells is significantly reduced during the early phase of the immune response following depletion of Langerin+ DCs. Consequently, the total number of activated CD8+ T cells within the draining lymph node and at the site of infection is diminished. Furthermore, we show that the impaired CD8+ T cell response is due to the absence of Langerin+ dDCs and not Langerhans cells. Nevertheless, the CD4+ T cell response is not altered and the infection is cleared as effectively in DT-treated Lang-DTR mice as in control mice. This clearly demonstrates that Langerin+ DCs are, in general, dispensable for an efficient adaptive immune response against L. major parasites. Thus, we propose a novel concept that, in the experimental model of leishmaniasis, priming of CD4+ T cells is mediated by Langerin− dDCs, whereas Langerin+ dDCs are involved in early priming of CD8+ T cells.

Identificador

http://pure.qub.ac.uk/portal/en/publications/priming-of-cd8-and-cd4-t-cells-in-experimental-leishmaniasis-is-initiated-by-different-dendritic-cell-subtypes(4b734ea9-993d-496a-925f-0548e2442773).html

http://dx.doi.org/10.4049/jimmunol.182.2.774

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Brewig , N , Kissenpfennig , A , Malissen , B , Veit , A , Bickert , T , Fleischer , B , Mostböck , S & Ritter , U 2009 , ' Priming of CD8+ and CD4+ T Cells in Experimental Leishmaniasis Is Initiated by Different Dendritic Cell Subtypes ' Journal of Immunology , vol 182 , no. 2 , pp. 774-783 . DOI: 10.4049/jimmunol.182.2.774

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/2400/2403 #Immunology #/dk/atira/pure/subjectarea/asjc/2700 #Medicine(all)
Tipo

article