Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets: Report of the BIOMED-2 Concerted Action BHM4-CT98-3936.


Autoria(s): Evans, P.A.; Van Dongen, J.J.M.; Groenen, P.J.T.A.; Ch, Pott; Salles, G.; Davi, F.; Berger, J.F.; Garcia, J.F.; Van Krieken, J.H.J.M.; Pals, S.; Kluin, P.; Schuuring, E.; Spaargaren, M.; Boone, E.; Gonzàlez, D.; Martinez, R.; Villuendas, R.; Gamerio, P.; Diss, T.C.; Mills, Ken; Morgan, G.J.; Carter, G.I.; Milner, B.J.; Pearson, D.; Hummel, M.; Jung, W.; Ott, M.; Canioni, D.; Beldjord, K.; Bastard, C.; Delfau-Larue, M.H.; Molina, T.J.; Cabeçadas, J.
Data(s)

01/01/2007

Resumo

Polymerase chain reaction (PCR) assessment of clonal immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements is an important diagnostic tool in mature B-cell neoplasms. However, lack of standardized PCR protocols resulting in a high level of false negativity has hampered comparability of data in previous clonality studies. In order to address these problems, 22 European laboratories investigated the Ig/TCR rearrangement patterns as well as t(14;18) and t(11;14) translocations of 369 B-cell malignancies belonging to five WHO-defined entities using the standardized BIOMED-2 multiplex PCR tubes accompanied by international pathology panel review. B-cell clonality was detected by combined use of the IGH and IGK multiplex PCR assays in all 260 definitive cases of B-cell chronic lymphocytic leukemia (n¼56), mantle cell lymphoma (n¼54), marginal zone lymphoma (n¼41) and follicular lymphoma (n¼109). Two of 109 cases of diffuse large B-cell lymphoma showed no detectable clonal marker. The use of these techniques to assign cell lineage should be treated with caution as additional clonal TCR gene rearrangements were frequently detected in all disease categories. Our study indicates that the BIOMED-2 multiplex PCR assays provide a powerful strategy for clonality assessment in B-cell malignancies resulting in high Ig clonality detection rates particularly when IGH and IGK strategies are combined.

Identificador

http://pure.qub.ac.uk/portal/en/publications/significantly-improved-pcrbased-clonality-testing-in-bcell-malignancies-by-use-of-multiple-immunoglobulin-gene-targets-report-of-the-biomed2-concerted-action-bhm4ct983936(bc305c80-1401-4b4c-8ed7-352214a804bf).html

http://dx.doi.org/10.1038/sj.leu.2404479

http://www.scopus.com/inward/record.url?scp=33846483156&partnerID=8YFLogxK

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Evans , P A , Van Dongen , J J M , Groenen , P J T A , Ch , P , Salles , G , Davi , F , Berger , J F , Garcia , J F , Van Krieken , J H J M , Pals , S , Kluin , P , Schuuring , E , Spaargaren , M , Boone , E , Gonzàlez , D , Martinez , R , Villuendas , R , Gamerio , P , Diss , T C , Mills , K , Morgan , G J , Carter , G I , Milner , B J , Pearson , D , Hummel , M , Jung , W , Ott , M , Canioni , D , Beldjord , K , Bastard , C , Delfau-Larue , M H , Molina , T J & Cabeçadas , J 2007 , ' Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets: Report of the BIOMED-2 Concerted Action BHM4-CT98-3936. ' Leukemia , vol 21(2) , no. 2 , pp. 207-214 . DOI: 10.1038/sj.leu.2404479

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/2700/2720 #Hematology #/dk/atira/pure/subjectarea/asjc/1300/1306 #Cancer Research
Tipo

article