The production and characterisation of dinitrocarbanilide antibodies raised using antigen mimics.
| Data(s) |
01/06/2002
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|---|---|
| Formato |
application/pdf |
| Identificador |
http://dx.doi.org/10.1016/S0022-1759(02)00040-6 http://pure.qub.ac.uk/ws/files/422238/Nitroimidazole%20antibody%20production%20paper.pdf http://www.scopus.com/inward/record.url?scp=0036606114&partnerID=8YFLogxK |
| Idioma(s) |
eng |
| Direitos |
info:eu-repo/semantics/restrictedAccess |
| Fonte |
Fodey , T , Connolly , L , Elliott , C , Crooks , S R H & Delahaut , P 2002 , ' The production and characterisation of dinitrocarbanilide antibodies raised using antigen mimics. ' Journal of Immunological Methods , vol 264 , no. 1-2 , pp. 45-51 . DOI: 10.1016/S0022-1759(02)00040-6 |
| Palavras-Chave | #/dk/atira/pure/subjectarea/asjc/1300/1305 #Biotechnology #/dk/atira/pure/subjectarea/asjc/2400/2403 #Immunology |
| Tipo |
article |
| Resumo |
Polyclonal antibodies were produced to detect the coccidiostat nicarbazin. Due to structural constraints of the active component of nicarbazin, dinitrocarbanilide (DNC), three different compounds that shared a common substructure with DNC were used as antigen mimics. The compounds (N-suceinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide (SAN), L-glutamic acid gamma-(p-nitroanilide) (GAN) and p-nitrosuccinanilic acid (NSA)) were conjugated to a carrier protein and used in the immunisation of rabbits. Five different polyclonal sera were produced and consequently characterised. The antibodies exhibited an IC50 range of 2.3-7.6 ng/ml using a competitive ELISA procedure, Serum from one rabbit, R555, exhibited an IC50 of 2.9 ng/ml for DNC and cross-reactivity studies showed that this serum was specific for DNC and did not cross-react with other coccidiostats such as halofuginone, toltrazuril or ronidazole. (C) 2002 Elsevier Science B.V. All rights reserved. |
