Enhancement of Ca2+-dependent outward current in sheep bladder myocytes by evans blue dye.


Autoria(s): Hollywood, M.A.; McCloskey, Karen; McHale, N.G.; Thornbury, K.D.
Data(s)

1998

Resumo

Whole-cell and inside-out patch-clamp techniques were used to assess the action of a well-known dye, Evans blue, on membrane currents in bladder isolated smooth muscle cells from sheep. In whole cells Evans blue dose-dependently increased the outward current by up to fivefold. In contrast, Evans blue had no effect on inward Ca2+ current. The effect on outward current was abolished or reduced if the cells were bathed in Ca2+-free solution, iberiotoxin (5 x 10(-8) M), or charybdotoxin (5 x 10(-8) M), but was unaffected by externally applied caffeine (5 mM) or in cells exposed to heparin (1 mg/ml) via the patch pipette. In inside-out patches bathed in a Ca2+ concentration of 5 x 10(-7) M, Evans blue (10(-4) M) increased the open probability of large-conductance (298-pS) Ca2+-dependent K+ channels (BK channels), shifting the half maximal-activation voltage by -70 mV. We conclude that Evans blue dye acts as an opener of BK channels.

Identificador

http://pure.qub.ac.uk/portal/en/publications/enhancement-of-ca2dependent-outward-current-in-sheep-bladder-myocytes-by-evans-blue-dye(ee2cdf5e-7f31-479f-8659-4a627961cfdc).html

http://www.scopus.com/inward/record.url?scp=0032037589&partnerID=8YFLogxK

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Hollywood , M A , McCloskey , K , McHale , N G & Thornbury , K D 1998 , ' Enhancement of Ca2+-dependent outward current in sheep bladder myocytes by evans blue dye. ' Pflugers Archiv : European journal of physiology , vol 435(5) , no. 5 , pp. 631-636 .

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/1300/1314 #Physiology
Tipo

article