Bioreactor Expansion of Human Adult Bone Marrow-Derived Mesenchymal Stem Cells


Autoria(s): Chen, X.; Xu, Haibo; Wan, Chao; McCaigue, Mervyn; Li, Gang
Data(s)

01/09/2006

Resumo

Supplementation of mesenchymal stem cells (MSCs) during hematopoietic stem cell transplantation (HSCT) alleviates complications such as graft-versus-host disease, leading to a speedy recovery of hematopoiesis. To meet such clinical demand, a fast MSCs expansion method is required. In the present study, we examined the feasibility of expanding MSCs from the isolated bone marrow mononuclear cells using a rotary bioreactor system. The cells were cultured in a rotary bioreactor with Myelocult� medium containing a combination of supplementary factors, including stem cell factor (SCF), interleukin 3 and 6 (IL-3, IL-6). After 8 days of culture, total cell numbers, Stro-1+CD44+CD34- MSCs and CD34+CD44+Stro-1- HSCs were increased 9, 29, and 8 folds respectively. Colony forming efficiency-fibroblast per day (CFE-F/day) of the bioreactor-treated cells was 1.44-fold higher than that of the cells without bioreactor treatment. The bioreactor-expanded MSCs showed expression of primitive MSCs markers endoglin (SH2) and vimentin, whereas markers associated with lineage differentiation including osteocalcin (osteogenesis), Type II collagen (chondrogenesis) and C/EBPα (adipogenesis) were not detected. Upon induction, the bioreactor-expanded MSCs were able to differentiate into osteoblasts, chondrocytes and adipocytes. Taken together, we conclude that the rotary bioreactor with the modified Myelocult� medium reported in this study may be used to rapidly expand MSCs.

Identificador

http://pure.qub.ac.uk/portal/en/publications/bioreactor-expansion-of-human-adult-bone-marrowderived-mesenchymal-stem-cells(551cd3d8-1d5b-4429-9579-c2480860b96c).html

http://dx.doi.org/10.1634/stemcells.2005-0591

http://www.scopus.com/inward/record.url?scp=33749188522&partnerID=8YFLogxK

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Chen , X , Xu , H , Wan , C , McCaigue , M & Li , G 2006 , ' Bioreactor Expansion of Human Adult Bone Marrow-Derived Mesenchymal Stem Cells ' Stem Cells , vol 24(9) , no. 9 , pp. 2052-2059 . DOI: 10.1634/stemcells.2005-0591

Palavras-Chave #/dk/atira/pure/subjectarea/asjc/1300/1307 #Cell Biology
Tipo

article