Pathogenicity of Salmonella: SopE-mediated membrane ruffling is independent of inositol phosphate signals


Autoria(s): Deleu, Sandrine; Choi, Kuicheon; Reece, Jeff M.; Shears, Stephen B
Data(s)

2006

Resumo

Studies [Zhou, D. Chen, L.-M. Hernandez, L. Shears, S.B. and Galán, J.E. (2001) A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host-cell actin cytoskeleton rearrangements and bacterial internalization. Mol. Microbiol. 39, 248-259] with engineered Salmonella mutants showed that deletion of SopE attenuated the pathogen's ability to deplete host-cell InsP5 and remodel the cytoskeleton. We pursued these observations: In SopE-transfected host-cells, membrane ruffling was induced, but SopE did not dephosphorylate InsP5, nor did it recruit PTEN (a cytosolic InsP5 phosphatase) for this task. However, PTEN strengthened SopE-mediated membrane ruffling. We conclude SopE promotes host-cell InsP5 hydrolysis only with the assistance of other Salmonella proteins. Our demonstration that Salmonella-mediated cytoskeletal modifications are independent of inositolphosphates will focus future studies on elucidating alternate pathogenic consequences of InsP5 metabolism, including ion channel conductance and apoptosis.

info:eu-repo/semantics/published

Formato

1 full-text file(s): application/pdf

Identificador

uri/info:doi/10.1016/j.febslet.2006.02.019

uri/info:pii/S0014-5793(06)00206-7

uri/info:pmid/16500648

https://dipot.ulb.ac.be/dspace/bitstream/2013/229470/3/Elsevier_213097.pdf

http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/229470

Idioma(s)

en

Fonte

FEBS letters, 580 (7

Palavras-Chave #Sciences bio-médicales et agricoles #Acid Anhydride Hydrolases #Actins -- metabolism #Bacterial Proteins -- genetics -- physiology #Cell Line, Tumor #Cell Membrane -- microbiology #Cytoskeleton -- metabolism #Humans #Inositol Phosphates -- physiology #PTEN Phosphohydrolase -- pharmacology #Salmonella -- pathogenicity #Salmonella Infections #Signal Transduction #Transfection #Virulence
Tipo

info:eu-repo/semantics/article

info:ulb-repo/semantics/articlePeerReview

info:ulb-repo/semantics/openurl/article

Direitos

1 full-text file(s): info:eu-repo/semantics/restrictedAccess