Regulation of the alpha-fetoprotein promoter: Ku binding and DNA spatial conformation
| Data(s) |
2006
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|---|---|
| Resumo |
This work shows that the proximal promoter of the mouse Afp gene contains a Ku binding site and that Ku binding is associated with down-regulation of the transcriptional activity of the Afp promoter. The Ku binding site is located in a segment able to adopt a peculiar structured form, probably a hairpin structure. Interestingly, the structured form eliminates the binding sites of the positive transcription factor HNF1. Furthermore, a DNAse hypersensitive site is detected in footprinting experiments done with extracts of AFP non-expressing hepatoma cells. These observations suggest that the structured form is stabilised by Ku and is associated with extinction of the gene in AFP non-expressing hepatic cells. Journal Article Research Support, Non-U.S. Gov't info:eu-repo/semantics/published |
| Formato |
1 full-text file(s): application/pdf |
| Identificador |
uri/info:doi/10.1016/j.biochi.2006.05.006 uri/info:pii/S0300-9084(06)00081-2 uri/info:pmid/16765502 local/cs-0004 https://dipot.ulb.ac.be/dspace/bitstream/2013/17859/1/Elsevier_5674.pdf http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/17859 |
| Idioma(s) |
en |
| Direitos |
1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| Fonte |
Biochimie, 88 (10 |
| Palavras-Chave | #Biologie moléculaire #Animals #Antigens, Nuclear -- metabolism #Base Sequence #Binding Sites #Cell Line, Tumor #DNA -- chemistry #DNA -- metabolism #DNA-Binding Proteins -- metabolism #Gene Expression Regulation #Hepatocyte Nuclear Factor 1 #Humans #Mice #Molecular Sequence Data #Nucleic Acid Conformation #Promoter Regions, Genetic #Rats #alpha-Fetoproteins -- genetics #alpha-Fetoproteins -- metabolism #Alpha-fetoprotein #DNA conformation #Ku #Transcription |
| Tipo |
info:eu-repo/semantics/article info:ulb-repo/semantics/articlePeerReview info:ulb-repo/semantics/openurl/article |
