NgBR is essential for endothelial cell glycosylation and vascular development.


Autoria(s): Park, EJ; Grabińska, KA; Guan, Z; Sessa, WC
Cobertura

England

Data(s)

01/02/2016

Resumo

NgBR is a transmembrane protein identified as a Nogo-B-interacting protein and recently has been shown to be a subunit required for cis-prenyltransferase (cisPTase) activity. To investigate the integrated role of NgBR in vascular development, we have characterized endothelial-specific NgBR knockout embryos. Here, we show that endothelial-specific NgBR knockout results in embryonic lethality due to vascular development defects in yolk sac and embryo proper. Loss of NgBR in endothelial cells reduces proliferation and promotes apoptosis of the cells largely through defects in the glycosylation of key endothelial proteins including VEGFR2, VE-cadherin, and CD31, and defective glycosylation can be rescued by treatment with the end product of cisPTase activity, dolichol phosphate. Moreover, NgBR functions in endothelial cells during embryogenesis are Nogo-B independent. These data uniquely show the importance of NgBR and protein glycosylation during vascular development.

Formato

167 - 177

Identificador

http://www.ncbi.nlm.nih.gov/pubmed/26755743

embr.201540789

EMBO Rep, 2016, 17 (2), pp. 167 - 177

http://hdl.handle.net/10161/11565

1469-3178

Idioma(s)

ENG

Relação

EMBO Rep

10.15252/embr.201540789

Palavras-Chave #NgBR #cis‐prenyltransferase #dolichol #glycosylation #vascular development
Tipo

Journal Article