Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo.


Autoria(s): Juhas, M; Engelmayr, GC; Fontanella, AN; Palmer, GM; Bursac, N
Data(s)

15/04/2014

Formato

5508 - 5513

Identificador

http://www.ncbi.nlm.nih.gov/pubmed/24706792

1402723111

Proc Natl Acad Sci U S A, 2014, 111 (15), pp. 5508 - 5513

http://hdl.handle.net/10161/8413

1091-6490

Relação

Proc Natl Acad Sci U S A

10.1073/pnas.1402723111

Palavras-Chave #angiogenesis #contractile force #self-repair #tissue engineering #window chamber #Animals #Biomimetics #Cobra Cardiotoxin Proteins #Mice #Mice, Nude #Microvessels #Muscle Contraction #Muscle Development #Muscle, Skeletal #Tissue Engineering
Tipo

Journal Article

Cobertura

United States

Resumo

Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury. Here, we describe a platform for engineering and testing highly functional biomimetic muscle tissues with a resident satellite cell niche and capacity for robust myogenesis and self-regeneration in vitro. Using a mouse dorsal window implantation model and transduction with fluorescent intracellular calcium indicator, GCaMP3, we nondestructively monitored, in real time, vascular integration and the functional state of engineered muscle in vivo. During a 2-wk period, implanted engineered muscle exhibited a steady ingrowth of blood-perfused microvasculature along with an increase in amplitude of calcium transients and force of contraction. We also demonstrated superior structural organization, vascularization, and contractile function of fully differentiated vs. undifferentiated engineered muscle implants. The described in vitro and in vivo models of biomimetic engineered muscle represent enabling technology for novel studies of skeletal muscle function and regeneration.

Idioma(s)

ENG