Detection of amino-terminal extracellular domain of somatostatin receptor 2 by specific monoclonal antibodies and quantification of receptor density in medulloblastoma.


Autoria(s): Kuan, CT; Wikstrand, CJ; McLendon, RE; Zalutsky, MR; Kumar, U; Bigner, DD
Data(s)

01/12/2009

Formato

389 - 403

Identificador

http://www.ncbi.nlm.nih.gov/pubmed/20025498

Hybridoma (Larchmt), 2009, 28 (6), pp. 389 - 403

http://hdl.handle.net/10161/3241

1557-8348

Idioma(s)

ENG

en_US

Relação

Hybridoma (Larchmt)

10.1089/hyb.2009.0049

Hybridoma

Tipo

Journal Article

Cobertura

United States

Resumo

Somatostatin receptor 2 (SSTR2) is expressed by most medulloblastomas (MEDs). We isolated monoclonal antibodies (MAbs) to the 12-mer (33)QTEPYYDLTSNA(44), which resides in the extracellular domain of the SSTR2 amino terminus, screened the peptide-bound MAbs by fluorescence microassay on D341 and D283 MED cells, and demonstrated homogeneous cell-surface binding, indicating that all cells expressed cell surface-detectable epitopes. Five radiolabeled MAbs were tested for immunoreactive fraction (IRF), affinity (KA) (Scatchard analysis vs. D341 MED cells), and internalization by MED cells. One IgG(3) MAb exhibited a 50-100% IRF, but low KA. Four IgG(2a) MAbs had 46-94% IRFs and modest KAs versus intact cells (0.21-1.2 x 10(8) M(-1)). Following binding of radiolabeled MAbs to D341 MED at 4 degrees C, no significant internalization was observed, which is consistent with results obtained in the absence of ligand. However, all MAbs exhibited long-term association with the cells; binding at 37 degrees C after 2 h was 65-66%, and after 24 h, 52-64%. In tests with MAbs C10 and H5, the number of cell surface receptors per cell, estimated by Scatchard and quantitative FACS analyses, was 3.9 x 10(4) for the "glial" phenotype DAOY MED cell line and 0.6-8.8 x 10(5) for four neuronal phenotype MED cell lines. Our results indicate a potential immunotherapeutic application for these MAbs.

Palavras-Chave #Amino Acid Sequence #Antibodies, Monoclonal #Blotting, Western #Cell Line, Tumor #DNA Primers #Enzyme-Linked Immunosorbent Assay #Flow Cytometry #Humans #Immunohistochemistry #Immunotherapy #Medulloblastoma #Protein Structure, Tertiary #Receptors, Somatostatin #Reverse Transcriptase Polymerase Chain Reaction